文献类型：期刊文献

中文题名：柚木愈伤组织诱导培养的研究

英文题名：Research on induction and culture of callus of teak(Tectona grandis)

作者：郭彦彤[1] 梁坤南[1] 黄桂华[1] 曾炳山[1] 裘珍飞[1] 周再知[1]

第一作者：郭彦彤

机构：[1]中国林业科学研究院热带林业研究所

年份：2012

卷号：32

期号：3

起止页码：53-58

中文期刊名：中南林业科技大学学报

外文期刊名：Journal of Central South University of Forestry & Technology

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD_E2011_2012】；

基金：国家林业公益性行业科研专项重大项目(201104001)

语种：中文

中文关键词：柚木;茎段;组织培养;愈伤组织诱导

外文关键词：teak; stem; tissue culture; callus induction

分类号：S796;S722.37

摘要：以MS、1/2MS和WPM为基本培养基分别添加0.5mg.L-1NAA+1 mg.L-16-BA,以柚木无菌组培瓶苗的茎尖,带节间的茎段,不带节间茎段、叶片为外植体,分析不同类型培养基、外植体对柚木愈伤组织生长的影响;并以MS为基本培养基、茎段(不带节间)为外植体进行生长调节剂水平、固液培养方式、光照条件及培养时间试验,探讨柚木愈伤组织生长的最佳培养条件。结果表明:MS培养基适合愈伤组织生长,外植体茎段(不带节间)为较适外植体,较适生长调节剂浓度组合为0.5 mg.L-1NAA+1 mg.L-16-BA及2 mg.L-1NAA+2 mg.L-16-BA,暗培养28 d后转到光下培养的愈伤诱导率最高,滤纸桥液体培养方式有利于愈伤的诱导,愈伤组织最适继代时间为30～35 d。
Adopting the Ms, 1/2 Ms, and WPM as different basic mediums supplemented with 0.5mg·L^-1NAA＋1mg·L^-1 6-BA respectively, the various explants of node stem, top buds, stem （withou~ internode） and leaves of tissue-culture container seedlings of teak （Tectona grandis L.f.）, were used to study the effects of different mediums on callus growth of teak. Growth regulators, solid and liquid cultivation mode, light conditions and culture time were studied using MS as basic medium and stem （without intemode） as explants to explore the best culture conditions of the callus growth..The results show that MS medium was best for callus growth. The growth regulator combinations of 0.5 mg·L^-1 NAA＋I mg·L^-1-BA and 2 mg·L^-1 NAA＋2 mg·L^-1 6-BA were suitable for callus growth of teak. The explants cultured 28 days in dark condition then turned to light condition had higher rate of the callus induction. Filter paper bridge with MS liquid medium was better than solid culture medium. The optimal duration for callus subculture was around 30- 35 days.