文献类型：期刊文献

英文题名：Evolutionary history of PEPC genes in green plants: Implications for the evolution of CAM in orchids

作者：Deng, Hua[1,2] Zhang, Liang-Sheng[3,4] Zhang, Guo-Qiang[3,4] Zheng, Bao-Qiang[1] Liu, Zhong-Jian[3,4,5,6] Wang, Yan[1]

第一作者：邓华

通信作者：Liu, ZJ[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Forestry, Key Lab Tree Breeding & Cultivat,State Forestry A, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry Policy & Informat, Beijing 100091, Peoples R China;[3]Natl Orchid Conservat Ctr China, Shenzhen Key Lab Orchid Conservat & Utilizat, Shenzhen, Peoples R China;[4]Orchid Conservat & Res Ctr Shenzhen, Shenzhen, Peoples R China;[5]Tsinghua Univ, Ctr Biotechnol & BioMed, Grad Sch Shenzhen, Shenzhen 518057, Peoples R China;[6]South China Agr Univ, Coll Forestry, Guangzhou, Guangdong, Peoples R China

年份：2016

卷号：94

起止页码：559-564

外文期刊名：MOLECULAR PHYLOGENETICS AND EVOLUTION

收录：;Scopus(收录号：2-s2.0-84948965717);WOS:【SCI-EXPANDED(收录号:WOS:000366443300009)】；

基金：This work was supported by the Basic Research Fund of RIF (Grant No. RIF2014-05) and Development Special Fund of Biological Industry of Shenzhen Municipality (Grant No. JC201005310690A). We thank Ying-Qiu Tian and Jie Huang for help with lab work and Chao Bian for sharing bioinformatic resources and discussion.

语种：英文

外文关键词：Crassulacean acid metabolism (CAM); Orchidaceae; Phosphoenolpyruvate carboxylase (PEPC); Phylogeny; RNA-Seq sequences

摘要：The phosphoenolpyruvate carboxylase (PEPC) gene is the key enzyme in CAM and C-4 photosynthesis. A detailed phylogenetic analysis of the PEPC family was performed using sequences from 60 available published plant genomes, the Phalaenopsis equestris genome and RNA-Seq of 15 additional orchid species. The PEPC family consists of three distinct subfamilies, PPC-1, PPC-2, and PPC-3, all of which share a recent common ancestor in chlorophyte algae. The eudicot PPC-1 lineage separated into two clades due to whole genome duplication (WGD). Similarly, the monocot PPC-1 lineage also divided into PPC-1M1 and PPC-1M2 through an ancient duplication event. The monocot CAM- or C-4-related PEPC originated from the clade PPC-IMI. WGD may not be the major driver for the performance of CAM function by PEPC, although it increased the number of copies of the PEPC gene. CAM may have evolved early in monocots, as the CAM-related PEPC of orchids originated from the monocot ancient duplication, and the earliest CAM-related PEPC may have evolved immediately after the diversification of monocots, with CAM developing prior to C-4. Our results represent the most complete evolutionary history of PEPC genes in green plants to date and particularly elucidate the origin of PEPC in orchids. (C) 2015 The Authors. Published by Elsevier Inc.