文献类型：期刊文献

中文题名：基于种子无菌苗的云南甜龙竹组培快繁研究

英文题名：A Study of Tissue Culture and Rapid Propagation of Dendrocalamus brandisii Based on Embryo Sterile Seedlings

作者：郑祥亁[1] 陈凌娜[1] 孙茂盛[2] 崔永忠[1] 杨汉奇[1]

第一作者：郑祥亁

机构：[1]中国林业科学研究院资源昆虫研究所,昆明650233;[2]西南林业大学竹藤研究所,昆明650233

年份：2019

卷号：17

期号：4

起止页码：26-29

中文期刊名：世界竹藤通讯

外文期刊名：World Bamboo and Rattan

基金：西藏自治区科学技术厅项目(XZ201801-GA-11)

语种：中文

中文关键词：云南甜龙竹;种子;快速繁殖;组织培养

外文关键词：Dendrocalamus brandisii;seed;rapid propagation;tissue culture

分类号：S68

摘要：云南甜龙竹(Dendrocalamus brandisii)是优异的笋材两用大型丛生竹种,但常规无性繁殖技术培育的苗木因价格高而极大地阻碍了该竹种的推广栽培。文章以云南甜龙竹种子萌发的无菌苗为外植体,研究植物生长调节剂对试管苗快速繁殖及生根的影响。结果表明:丛生芽诱导的最佳培养基为MS+6-苄氨基腺嘌呤(6-BA)3.0 mg/L+萘乙酸(NAA)0.7 mg/L,诱导率可达86.33%;最佳继代增殖培养基为MS+NAA 0.3 mg/L+6-BA 6.0 mg/L+噻苯隆(TDZ)0.001 mg/L,增殖系数可达4.90;最佳生根培养基为1/2MS+NAA 2.0 mg/L+吲哚丁酸(IBA)4.0 mg/L,生根率可达71.45%。研究结果可为云南甜龙竹工厂化育苗提供技术支撑。
Dendrocalamus brandisii(Munro)Kurz is an excellent species of giant woody sympodial bamboo for edible shoots and timber purposes,but the high price of seedlings from the conventional asexual propagation prevents it from the large-scale cultivation.To develop the tissue culture system of D.brandisii,the effects of plant growth regulators on rapid propagation and rooting in vitro are studied using the sterile seedling sprouting from seeds as explants.The results show that the optimal medium for axillary bud induction is MS+6-BA 3.0 mg·L^-1+NAA 0.7 mg·L^-1,with a 86.33%germination rate of axillary buds;the suitable medium for multiplication is MS+NAA 0.3 mg·L^-1+6-BA 6 mg·L^-1+TDZ 0.001 mg·L^-1,and the multiplication coefficient can reach up to 4.90;the optimized medium for rooting is 1/2MS+NAA 2.0 mg·L^-1+IBA 4.0 mg·L^-1,with a 71.45%rooting rate.This study provides technical support for industrial seedling production of D.brandisii.