文献类型：期刊文献

中文题名：烂皮病菌侵染对新疆杨光合特性及碳水代谢的影响

英文题名：Effects of Valsa sordida Infection on Photosynthetic Characteristics and Carbon-Water Metabolism in Populus alba var.pyramidalis

作者：李金鑫[1] 张一南[1] 苗瑞芬[2] 邢军超[1] 李敏[1] 申宛娜[1] 王黎[3] 赵嘉平[1]

第一作者：李金鑫

机构：[1]中国林业科学研究院林业新技术研究所,北京100091;[2]内蒙古五原县林业和草原局种苗站,内蒙古巴彦淖尔015100;[3]中国林业科学研究院林业研究所,北京100091

年份：2021

卷号：34

期号：5

起止页码：58-68

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2019SZ010)。

语种：中文

中文关键词：烂皮病菌(Valsa sordida);气体交换参数;叶绿素荧光参数;光响应曲线;非结构性碳水化合物;水分利用效率

外文关键词：Valsa sordida;gas-exchange;chlorophyll fluorescence;photosynthetic light-response curve;non-structural carbohydrates;water use efficiency

分类号：S763.15

摘要：[目的]研究烂皮病菌侵染对新疆杨叶片光合响应以及水分代谢特征的影响,探讨病菌侵染下杨树光合作用与水分代谢之间的相关性,为杨树烂皮病的发生及控制提供理论及实验依据。[方法]以1年生新疆杨为植物材料,采用枝干表皮微环割方法接种烂皮病菌,研究烂皮病菌侵染10~30天新疆杨的叶片气体交换、叶绿素荧光、根部非结构性碳水化合物以及叶片正午水势等指标,分析蒸腾速率、正午水势分别与气孔导度以及水汽压亏缺之间的关系。[结果]与环割对照相比,烂皮病菌侵染显著降低新疆杨叶片的净光合速率(62.45%~91.05%)、气孔导度(64.19%~87.43%)、光系统Ⅱ的最大光化学效率(19.13%~42.79%)、实际光化学效率(46.04%~69.93%)、电子传递速率(52.58%~68.03%)等参数;显著抑制新疆杨叶片最大净光合速率(76.94%)、光饱和点(40.40%)及表观量子效率(46.09%),并显著升高暗呼吸速率(82.14%)及光补偿点(242.42%)。烂皮病菌侵染显著降低根部可溶性糖(35.06%~44.50%,20~30天)及淀粉含量(35.77%~58.39%,10~30天)。烂皮病菌侵染显著抑制叶片的蒸腾速率(57.36%~80.49%)、水分利用效率(24.92%~70.55%)以及升高水汽压亏缺(13.59%~33.65%)和叶片正午水势(39.74%,20天)。相关性分析结果表明蒸腾速率与气孔导度呈正相关,与水汽压亏缺呈负相关;叶片正午水势与气孔导度及水汽压亏缺均无线性关系,烂皮病菌侵染导致的气孔关闭与叶片水分状况无关。[结论]烂皮病菌侵染下新疆杨叶片净光合速率降低的主要原因是叶片光能转化、光合电子传递及光能利用受阻。烂皮病菌侵染并未造成新疆杨叶部水分胁迫,甚至有一定的改善作用;同时,影响寄主根系的碳积累,导致根部非结构性碳水化合物含量始终在胁迫初期时的水平。
[Objective]The photosynthetic response and water metabolism characteristics of P.alba var.pyramidalis leaves were studied,and the correlation between photosynthesis and water metabolism of poplar under pathogen infection was discussed in this study,so as to provide theoretical and experimental basis for the occurrence and control of the poplar Valsa canker disease.[Method]Using a girdling-inoculation system,we evaluated gas-exchange and chlorophyll fluorescence characteristics,concentrations of non-structural carbohydrates in root and midday water potential of one-year poplar saplings with Valsa canker disease,and analyzed the relationship between transpiration rate,midday water potential and stomatal conductance,vapor pressure deficit.[Result]Compared with girdle control,Valsa canker significantly reduced the net photosynthetic rate(62.45%to 91.05%),stomatal conductance(64.19%to 87.43%),the maximum photochemical efficiency(19.13%to 42.79%),actual photochemical efficiency(4.04%to 69.93%)and electron transport rate(52.58%to 68.03%);also decreased the maximum net photosynthetic rate(76.94%),light saturation point(40.40%)and apparent quantum efficiency(46.09%),and increased dark respiration rate(82.14%)and light compensation point(242.42%).Valsa canker infection significantly decreased soluble sugar(35.06%to 44.50%,20-30 day)and starch content(35.77%to 58.39%,10-30 day)in roots.The fungi inhibited leaf transpiration rate(57.36%to 80.49%),water use efficiency(24.92%to 70.55%),and increased water vapor pressure deficit(13.59%to 33.65%)and midday water potential(39.74%,20 day).The results of correlation analysis showed that transpiration rate was positively correlated with stomatal conductance and negatively correlated with water vapor pressure deficit,and there was no linear relationship between midday water potential and stomatal conductance and water vapor pressure deficit.Stomatal closure caused by Valsa canker infection was not related to leaf water status.[Conclusion]The main reasons for the decrease of net photosynthetic rate of poplar leaves were that leaf light energy conversion,photosynthetic electron transport and light energy utilization were hindered.Valsa infection did not cause water stress,even had some improvement.And had an important effect on the carbon accumulation of host roots,leading to the content of non-structural carbohydrates in roots was always at the initial level of infection.