文献类型：期刊文献

英文题名：Efficient production of isoquercitin, icariin and icariside II by a novel thermostable a-l-rhamnosidase PodoRha from Paenibacillus odorifer with high a-1, 6-/a-1, 2-glycoside specificity

作者：Xie, Jingcong[1,2] Zhao, Jian[1,2] Zhang, Ning[1,2] Xu, Hao[1,2] Yang, Jing[1,2] Ye, Jun[1,2] Jiang, Jianchun[1,2]

第一作者：Xie, Jingcong

通信作者：Xie, JC[1];Zhang, N[1];Xie, JC[2];Zhang, N[2]

机构：[1]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Natl Engn Lab Biomass Chem Utilizat, Key & Open Lab Forest Chem Engn, Nanjing 210042, Peoples R China;[2]Nanjing Forestry Univ, Coinnovat Ctr Efficient Proc & Utilizat Forest Res, Nanjing 210037, Peoples R China

年份：2022

卷号：158

外文期刊名：ENZYME AND MICROBIAL TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000804813400002)】；

基金：This work was financially supported by Fundamental Research funds of CAF (CAFYBB2018GD002-04) , National Natural Science Foundation of China (No. 32101477) , Jiangsu Key Laboratory of Biomass Energy and Material (No. JSBEM-S-201802) , and National Natural Science Foundation of China (No. 31770636) .

语种：英文

外文关键词：Flavonoid bioconversion; a-l-rhamnosidase; Thermostability; Isoquercitin; Icariin; Icariside II

摘要：Minor flavonoids, such as isoquercitin, icariin and icariside II, have desired bioactivity and bioavailability. To enhance the production efficiency on converting rutin and epimedin C into minor flavonoids, a novel GH family 78 alpha-L-rhamnosidase PodoRha from the Paenibacillus odorifer was cloned and heterologous over-expressed in E. coli BL21(DE3). PodoRha exhibited a high catalytic ability in cleaving outer alpha-1, 2-rhamnopyranosyl moieties of epimedin C and alpha-1, 6-rhamnopyranosyl of rutin. It displayed the highest activity at 45 ? and pH 6.5, and has great thermostability at 40 ?. Under the optimal transformation condition (40 ?, pH 6.5, 1 h incubation), 10 g/L of Rutin was 99.86% transformed into 6.95 g/L of isoquercitin by PodoRha with a crude productivity of 6.95 g.L-1.h(-1); 1.964 g/L icariin was detected after the epimedin C of the total Epimedium flavonoids (10 g/L) were 99.98% converted by PodoRha. Moreover, by using a two-stage conversion system with a crude icariside II productivity of 2.081 g.L-1.h(-1), 5 g/L epimedin C was 99.85% converted into 3.122 g/L of icariside II with 1 h incubation under pH 6.5 at 40 ? after addition of 5.5 U/mL PodoRha; then with 30 min incubation under pH 6.5 at 90 ? after addition of 0.8 U/mL IagBgl1.