文献类型：期刊文献

英文题名：An efficient material (Mn-Cu-AC/cellulose beads) for immobilizing laccase: excellent activity, high stability and effective pollutant removal rate

作者：Zhang, Yuying[1] Li, Na[1] Chen, Lianmei[1] Yu, Fangrui[1] Sheng, Xueru[1] Zhang, Jian[1] Ping, Qingwei[1] Li, Hongbin[2]

第一作者：Zhang, Yuying

通信作者：Li, N[1];Li, HB[2]

机构：[1]Dalian Polytech Univ, Liaoning Prov Key Lab Pulp & Papermaking Engn, Dalian L16034, Peoples R China;[2]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Jiangsu Prov Key Lab Biomass Energy & Mat, Nanjing 210042, Peoples R China

年份：2024

卷号：48

期号：43

起止页码：18443-18455

外文期刊名：NEW JOURNAL OF CHEMISTRY

收录：;EI(收录号：20244417296614);Scopus(收录号：2-s2.0-85207397919);WOS:【SCI-EXPANDED(收录号:WOS:001336751900001)】；

基金：This work was financially supported by the Scientific Research Project of Education Department of Liaoning Province (LJKMZ20220885).

语种：英文

外文关键词：Manganese alloys - pH

摘要：Laccase immobilization is an effective way to realize the industrial application of laccase, but the loss of immobilized laccase activity is serious, which is an urgent problem to be solved. In this study, a novel laccase immobilization material was prepared by mixing waste Mn-Cu-loaded activated carbon with cellulose solution and regenerated in hydrochloric acid solution, which significantly improved the immobilized laccase activity. The optimum immobilization conditions (T, pH and initial laccase concentration) of Mn-Cu-AC/cellulose beads (Mn-Cu-ACC) for laccase were studied by the response surface method. Under the optimum conditions (T = 43 degrees C, pH = 4.0 and c = 6.8 g L-1), immobilized laccase (Laccase@Mn-Cu-ACC) showed excellent performance in terms of pH stability, thermostability and storage stability. And the enzyme activity of the laccase immobilized on the Mn-Cu-ACC carrier remarkably increased by 29.6% compared to that of the ACC. At last, the Laccase@Mn-Cu-ACC was used for the degradation of bisphenol A (BPA). Under the optimum conditions (t = 5.5 h, T = 45 degrees C, pH = 4.0 and initial cBPA = 70 mg L-1), the removal rate of BPA reached 72.3% in the first cycle, and after 5 cycles of reusing the immobilized laccase, the relative removal rate of BPA was retained at 53.2% of the initial removal rate. This study developed a new type of immobilized laccase carrier and provided a new path for the application of waste heavy metal loaded activated carbon. A novel immobilized laccase carrier was prepared using waste Mn-Cu-loaded activated carbon powder. Due to the existence of Mn-Cu dual ions, the immobilized laccase showed excellent application stability and performance in BPA removal.