文献类型：期刊文献

英文题名：De Novo Sequencing and Assembly Analysis of Transcriptome in Pinus bungeana Zucc. ex Endl

作者：Cai, Qifei[1,2,3] Li, Bin[1,2,3] Lin, Furong[1,2,3] Huang, Ping[1,2,3] Guo, Wenying[1,2,3] Zheng, Yongqi[1,2,3]

第一作者：Cai, Qifei

通信作者：Zheng, YQ[1];Zheng, YQ[2];Zheng, YQ[3]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, Key Lab Tree Breeding & Cultivat, State Forestry Adm, Beijing 100091, Peoples R China

年份：2018

卷号：9

期号：3

外文期刊名：FORESTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000428508200059)】；

基金：We are grateful to the supporting funding from the National Natural Science Foundation of China (31370667), National Key Technology of China (2013BA001B06) and Inner Mongolia Hesheng Institute of Ecological Sciences & Technology (Hesheng2014).

语种：英文

外文关键词：De novo; Pinus bungeana Zucc. ex Endl.; transcriptome; assembly analysis

摘要：To enrich the molecular data of Pinus bungeana Zucc. ex Endl. and study the regulating factors of different morphology controled by apical dominance. In this study, de novo assembly of transcriptome annotation was performed for two varieties of Pinus bungeana Zucc. ex Endl. that are obviously different inmorphology. More than 147 million reads were produced, which were assembled into 88,092 unigenes. Based on a similarity search, 11,692 unigenes showed significant similarity to proteins from Picea sitchensis (Bong.) Carr. From this collection of unigenes, a large number of molecular markers were identified, including 2829 simple sequence repeats (SSRs). A total of 158 unigenes expressed differently between two varieties, including 98 up-regulated and 60 down-regulated unigenes. Furthermore, among the differently expressed genes (DEGs), five genes which may impact the plant morphology were further validated by reverse transcription quantitative polymerase chain reaction (RT-qPCR). The five genes related to cytokinin oxidase/dehydrogenase (CKX), two-component response regulator ARR-A family (ARR-A), plant hormone signal transduction (AHP), and MADS-box transcription factors have a close relationship with apical dominance. This new dataset will be a useful resource for future genetic and genomic studies in Pinus bungeana Zucc. ex Endl.