文献类型：期刊文献

中文题名：厚垣普可尼亚菌液体发酵动力学

英文题名：Kinetic Study of the Liquid Fermentation for Pochonia chlamydosporia

作者：王曦茁[1] 马建伟[1] 汪来发[1] 李永[1] 余明忠[2]

第一作者：王曦茁

机构：[1]中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室;[2]四川省茂县林业局

年份：2014

卷号：50

期号：11

起止页码：75-81

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：科技部农业科技成果转化资金项目(2010GB24320615);国家微生物资源平台项目(NIMR2013-7)

语种：中文

中文关键词：厚垣普可尼亚菌;液体发酵;动力学模型

外文关键词：Pochonia chlamydosporia;liquid fermentation;kinetic model

分类号：S763.18

摘要：厚垣普可尼亚菌是对植物线虫有防治潜力的生防菌。利用正交设计优化厚垣普可尼亚菌液体发酵条件,利用Grow/Sigmoidal模型中的非线性拟合方程Logistic函数和Boltzmann函数构建该菌液体发酵过程中的孢子增长、底物消耗和产物生成的液体发酵动力学模型,并运用Origin Pro V8.6软件拟合求解出各模型参数。结果表明:在28℃条件下和500 mL摇瓶中,厚垣普可尼亚菌优化培养基及培养条件是葡萄糖15.0 g·L-1,玉米粉15.0g·L-1,大豆粉20.0 g·L-1,KH2PO40.25 g·L-1,MgSO4·7 H2O 0.25 g·L-1,接种量5%,初始pH 5.0,装量40%和转速190 r·min-1。厚垣普可尼亚菌液体发酵动力学模型可描述发酵过程中孢子含量、还原糖和氨基氮浓度随时间的变化规律,并对碳氮代谢进行分析。模型能够较好地拟合发酵过程:孢子量比生长速率在88.1 h时达到最大,最大值为0.030 2 h-1;还原糖比消耗速率在86.8 h时达到最大,最大值为0.033 4 h-1;氨基氮比生成速率在110.8 h时达到最大,最大值为6.864×10-4h-1。模型拟合和试验数据具有良好的适应性,可反映普可尼亚菌液体发酵过程的动力学特征,为其大规模生产奠定基础。
The fungus Pochonia chlamydosporia （ previously Verticillilum chlamydosporium ）, which has high parasitic rate on Meloidogyne incognita eggs,is a promising biological control agent for plant nematodes. The culture medium and condition of liquid fermentation of P. chlamydosporia were optimized with orthogonal design. At meantime,kinetic models of spore output and substrate consumption and amino nitrogen generation were established based on Logistic and Boltzmann Equation of Grow/Sigmoidal model and the parameters of the models were determined by Origin Pro V8. 6 software. The results showed that under the culture condition of temperature 28 ℃ and in 500 mL flasks,the optimal fermentation medium and culture condition were obtained,including glucose 15.0gL-1, corn flour 15. 0g·L^-1, soybean powder 20.0g·L^-1,KH2PO4 0.25g·L^-1,MgSO4·7 H2O 0.25g·L^-1,inoculum volume 5%（V/V）,the best initial pH value 5. 0,filling capacity 40%（V/V）and rotational speed 190 r min - 1. The maximum specific spore growth rate was 0. 030 2h-1 at 88. 1 h,specific reducing sugar consumption rate was 0. 0334h^-1 at 86. 8h and specific amino nitrogen generation rate was 6. 864 ·10 ^-4h^-1 at 110. 8 h. The model simulation fitted well with the experimental observations,which made it possible to reflect the kinetic characteristics of P. chlamydosporia during the liquid fermentation. These results could be employed for large scale production of P. chlamydosporia.