文献类型：期刊文献

中文题名：中国沙棘和云南沙棘的遗传分化及遗传多样性

英文题名：Genetic Differentiation and Genetic Diversity of Hippophae rhamnoides subsp.sinensis and H.rhamnoides subsp.yunnanensis

作者：王天翼[1] 徐悦[1] 王罗云[1] 张建国[1] 曾艳飞[1]

第一作者：王天翼

机构：[1]中国林业科学研究院林业研究所,国家林业和草原局林木培育重点实验室,北京100091

年份：2021

卷号：34

期号：4

起止页码：13-21

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：中国林业科学院基本科研业务费专项(CAFYBB2019ZY004)。

语种：中文

中文关键词：中国沙棘;云南沙棘;SSR;遗传分化;遗传多样性

外文关键词：Hippophae rhamnoides subsp.sinensis;Hippophae rhamnoides subsp.yunnanensis;SSR;genetic differentiation;genetic diversity

分类号：S722.3

摘要：[目的]探究中国沙棘和云南沙棘的遗传分化,明确二者分布区的地理边界,并评估其遗传多样性情况。[方法]利用18个简单重复序列(SSR)标记对32个中国沙棘和云南沙棘种群进行遗传变异检测,结合形态学和地理分布,利用系统发育分析和遗传结构分析鉴定种群分组情况,计算种群间遗传分化系数(FST),开展分子方差分析,计算遗传多样性参数(等位基因数(N_(A))、有效等位基因数(N_(E))、观测杂合度(H_(O))、期望杂合度(H_(E))和近交系数(F_(IS)))。[结果]中国沙棘的遗传多样性高于云南沙棘,中国沙棘种群间的遗传分化系数低于云南沙棘。AMOVA分析显示:种群间遗传变异所占比例中国沙棘(25.5%)低于云南沙棘(36.7%),表明来自不同地理种群的中国沙棘分化程度低、遗传变异更多的来源于种群内不同个体间。系统发育树分析显示:中国沙棘个体主要聚为一大支,而云南沙棘存在多个差异较大的分支,中国沙棘和四川地区的云南沙棘关系很近。遗传结构分析将所有中国沙棘和云南沙棘个体分为2组,2亚种间邻近种群存在杂交,尤其是位于四川北部的红原、松潘种群。[结论]中国沙棘相较于云南沙棘有更高的遗传多样性和较低的种群间分化;推测2亚种的分布边界位于四川北部的红原县、松潘县地区一带。
[Objective]To evaluate the genetic differentiation between Hippophae rhamnoides subsp.sinensis and H.rhamnoides subsp.yunnanensis,to clarify their geographic boundary in ambiguous areas,and estimate the genetic diversity within each taxon.[Method]18 Simple Sequence Repeat(SSR)makers were used to analyze the genetic variation of 32 H.rhamnoides subsp.sinensis and H.rhamnoides subsp.yunnanensis populations.Phylogenetic analysis and cluster analysis were conducted to identify the grouping of these populations according to their morphology and geographic distribution.The genetic differentiation coefficients were calculated among populations within each taxon,and the Analysis of Molecular Variance(AMOVA)was conducted to estimate the variance components at different levels.The genetic diversity parameters,such as allele number(N_(A)),effective allele number(N_(E)),observed heterozygosity(H_(O)),expected heterozygosity(H_(E))and inbreeding coefficient(F_(IS)),were calculated for each taxon and each population.[Result]The genetic diversity of H.rhamnoides subsp.sinensis was higher than that of H.rhamnoides subsp.yunnanensis.Compared with H.rhamnoides subsp.yunnanensis,H.rhamnoides subsp.sinensis had lower genetic differentiation coefficients between populations;and AMOVA analysis found that the percentage of variance among populations within taxa was lower in H.rhamnoides subsp.sinensis(25.5%)than in H.rhamnoides subsp.yunnanensis(36.7%),suggesting that the differentiation among geographical populations of H.rhamnoides subsp.sinensis was lower than that of H.rhamnoides subsp.yunnanensis,and the genetic variation of H.rhamnoides subsp.sinensis was mainly between individuals.Phylogenetic analysis suggested that all individuals of H.rhamnoides subsp.sinensis mainly clustered into one clade,while several highly differed clades were found in H.rhamnoides subsp.yunnanensis,and the subsp.sinensis was closer with Sichan subsp.yunnanensis clade than with others.By population genetic cluster analysis,all H.rhamnoides subsp.sinensis and H.rhamnoides subsp.yunnanensis individuals were divided into two major branches,and hybridization between them were found in several populations,especially in populations Hongyuan and Songpan that located in northern Sichuan province.[Conclusion]H.rhamnoides subsp.sinensis has higher genetic diversity and lower genetic differentiation than that of H.rhamnoides subsp.yunnanensis.Hongyuan and Songpan counties in northern Sichuan province are most likely to be the distribution boundary between H.rhamnoides subsp.sinensis and H.rhamnoides subsp.yunnanensis.