文献类型：期刊文献

中文题名：旱柳与龙爪柳α微管蛋白基因家族的克隆与序列分析

英文题名：Cloning and Sequence Analysis of α-Tubulin Gene Families in Salix matsudana Koidz. and S. matsudana var. tortuosa(Vilm.) Rehd

作者：睢金凯[1] 饶国栋[1,2] 张建国[1,2]

第一作者：睢金凯

机构：[1]国家林业局林木培育重点实验室中国林业科学研究院林业研究所;[2]南京林业大学南方现代林业协同创新中心

年份：2016

卷号：0

期号：9

起止页码：2305-2311

中文期刊名：分子植物育种

外文期刊名：Molecular Plant Breeding

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家自然科学基因青年项目(31400569)资助

语种：中文

中文关键词：α微管蛋白;序列相似性;系统发育;q;RT-PCR

外文关键词：α-tubulin;;Sequence similarity;;Phylogenetic;;qRT-PCR

分类号：R73

摘要：本研究以杨树和拟南芥α微管蛋白氨基酸序列为探针,利用本地化BLAST工具搜索柳树全基因组数据库,根据搜索所获序列克隆旱柳和龙爪柳α微管蛋白基因。通过q RT-PCR技术分析不同柳树组织中α微管蛋白基因的表达水平,并利用Bio Edit和MEGA5软件分别进行序列相似性和系统发育分析。结果显示,两种柳树各有8个α微管蛋白基因。柳树种内两两基因间核苷酸和氨基酸序列相似性分别在78.3%和88.4%以上,种间α微管蛋白氨基酸序列相似性在88.2%以上,而柳树与其它植物间的α微管蛋白氨基酸序列相似性在85.1%以上。我们还发现,半数α微管蛋白都包含一个C末端甲硫氨酸、亮氨酸、谷氨酸或谷氨酰胺,而不是更为典型的酪氨酸。在系统发育分析中α微管蛋白分为两类,说明这些基因可能起源于不同的祖先。C末端为Y型的TUA1基因在柳树一年生枝条基部、中段、伸长区和茎尖中高度表达,据此推测它们可能参与柳树木质部的发育过程。柳树TUA基因家族高度的序列相似性、C末端多样性和不同的表达模式可能赋予了细胞壁形成的灵活性,这对多年生木本植物的适应性具有重要意义。
In this study, the whole genome database of Salix was searched with α-tubulin amino acid sequences from Populus and Arabidopsis as probes by using a localized BLAST tool. The α-tubulin genes of S. matsudana and S. matsudana var. tortuosa were cloned based on the searched sequences. Expression level of the α-tubulin genes in various willow tissues were analyzed by q RT-PCR technology, and sequence similarity and phylogenetic analysis were carried out with Bio Edit and MEGA5 software, respectively. The results showed that each willow contained eight α-tubulin genes. Each α-tubulin gene shared more than 78.3% and 88.4% nucleotide and amino acid sequence similarity with one another in each willow species. And there was over 88.2% amino acid sequence similarity of the α-tubulin between the two willows. While the amino acid sequence similarity among Salix and other plants were larger than 85.1%. We also found that half of the eight Salix α-tubulin proteins contained a C-terminal methionine, leucine, glutamic acid or glutamine, instead of the more typical C-terminal tyrosine. In the phylogenetic analysis, α-tubulin proteins formed two distinct classes, which suggested these genes might originate from different ancestors. The C-terminal Y-type TUA1 genes were highly expressed in basal, middle, elongation and apical region of one year old branches of willows, therefore they were predicted to involve in the process of xylem development. The high degree of sequence similarity, C-terminal diversity and differential expressionpattern of Salix TUA gene families may confer flexibility in cell wall formation that is of important significance to the acclimatization of perennial woody plants.