文献类型：期刊文献

中文题名：小叶杨CCH基因的克隆及其在重金属胁迫下的表达模式

英文题名：Cloning and Expression Analysis of PsCCH from Populus simonii under Stresses of Heavy Metal

作者：刁姝[1] 苏晓华[1] 丁昌俊[1] 张冰玉[1]

机构：[1]林木遗传育种国家重点实验室中国林业科学研究院林业研究所国家林业局林木培育重点实验室,北京100091

年份：2015

卷号：25

期号：1

起止页码：32-36

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家高新技术研究发展计划(863计划)课题(2011AA100201)

语种：中文

中文关键词：小叶杨;铜伴侣蛋白;基因表达;重金属胁迫

外文关键词：Populus simonii; copper chaperone; gene expression; heavy metal stress

分类号：S718.6

摘要：利用生物信息学与分子生物学相结合的策略克隆了小叶杨CCH基因,基因编码区全长258 bp,编码由85个氨基酸残基构成的蛋白质,蛋白N端具有与铜离子结合的保守功能域MXCXXC,且具有铜伴侣蛋白典型的βαββαβ二级结构,命名为Ps CCH。系统进化分析表明:Ps CCH蛋白与橡胶、麻疯树、葡萄的CCH蛋白的亲缘关系最近(相似性90%93%)。通过实时定量PCR方法对其在不同浓度铜离子、其它重金属离子及植物生长激素处理下的表达模式进行了分析。结果表明:小叶杨Ps CCH的表达受多种重金属及植物激素的调控,在低浓度铜离子、铝离子、锌离子、水杨酸处理下,其mRNA表达先上升而后下降;而在高浓度铜离子、钴离子、汞离子、茉莉酸处理下则是持续下降。
The CCH gene from Populus simonii leaf was cloned according to a strategy combining bioinformatics and molecular biological approaches and its expression in response to heavy metals and plant growth regulators was detected by qPCR. This gene encodes a polypeptide of 85 amino acids, named as PsCCH. The deduced amino acid sequence of PsCCH possesses the conserved metal-binding motif in its N-terminal （ MXCXXC ） and has the classic βαββαβ-fold structure. Phylogeny analysis showed that the PsCCH was much closer to the CCH from Hevea bra- siliensis,Jatropha curcas and Vitis vinifera （with 90% -93% in amino acids similarity）. The results of qPCR showed that the expression of PsCCH was regulated by various heavy metals and plant growth regulators ： the PsCCH transcripts increased within one hour and then decreased to a lower level after 3 hours, such as in zinc, aluminum, low copper and jasmonic acid treatments; and the PsCCH transcripts continuously decreased during the treatment, such as in high copper, cobalt, mercury and salicylic acid treatments.