文献类型：期刊文献

中文题名：柑橘凤蝶非滞育蛹、滞育蛹与滞育蛹羽化成虫核酸含量比较

英文题名：Comparison of Nucleic Acid Contents among Non-diapause Pupae, Diapause Pupae and Eclosion-adult from Diapause Pupae of Papilio xuthus

作者：易传辉[1,2] 陈晓鸣[1] 史军义[1] 周成理[1]

第一作者：易传辉

机构：[1]中国林业科学研究院资源昆虫研究所/国家林业局资源昆虫培育与利用重点实验室;[2]云南林业职业技术学院

年份：2009

卷号：48

期号：5

起止页码：1160-1162

中文期刊名：湖北农业科学

外文期刊名：Hubei Agricultural Sciences

收录：CSTPCD;;北大核心:【北大核心2008】；

基金：国家林业局科技计划项目(2005-4-59;2008-4-68)

语种：中文

中文关键词：柑橘凤蝶;核酸含量;滞育;蛹;成虫

外文关键词：Papilio xuthus; nucleic acid content; diapause; pupae; adult

分类号：S436.669

摘要：为了深入了解柑橘凤蝶滞育机理,利用比色法测定了非滞育蛹、滞育蛹和滞育蛹羽化成虫不同发育阶段RNA和DNA含量。结果表明,非滞育蛹RNA含量在8.064 3～13.035 3μg.mg-1之间,滞育蛹在9.917 7～13.532 7μg.mg-1之间,羽化成虫初期为20.717 0μg.mg-1;非滞育蛹DNA含量在0.422 7～0.542 0μg.mg-1之间,滞育蛹在0.459 0～1.100 7μg.mg-1之间,羽化成虫初期为1.838 0μg.mg-1。滞育期间核酸含量较高,表明蛋白质代谢和细胞分裂较为活跃;核酸含量及变化与发育阶段有关。
In order to know the diapause mechanism of Papilio xuthus deeply, the nucleic acid content of non-diapause pupae, diapause pupae and eclosion-aduh from diapause pupae were measured by colorimetry, respectively. The results showed the RNA content of non-diapause pupae were between 8.064 3-13.035 3 μg·mg-1, diapause pupae were 9.9177-13.5327 μg·mg-1, and eclosion-aduh from diapause pupae was 20.717 0 μg·mg-1. The DNA content of non-diapause pupae were 0.422 7- 0.5420 μg·mg-1, diapause pupae were 0.4590～1.1007 μg·mg-1, and eclosion-aduh from diapause pupae was 1.838 0 μg·mg-1. The high content of nucleic acid in the stage of diapause revealed that the metabolism of protein and cell division was more active. The variance of nucleic acid was related to the stage of development of P. xuthus.