文献类型：期刊文献

中文题名：金花茶和白色山茶及其3个杂交品种类黄酮成分与花色的关系

英文题名：Flavonoid Components and Their Effects on Flower Colors in Camellia nitidissima,White C. japonica and Their Three Hybrid Cultivars

作者：李辛雷[1] 王佳童[1,2] 孙振元[2] 王洁[1] 殷恒福[1] 范正琪[1] 李纪元[1]

第一作者：李辛雷

机构：[1]中国林业科学研究院亚热带林业研究所,杭州311400;[2]林木遗传育种国家重点实验室,中国林业科学研究院林业研究所,北京100091

年份：2019

卷号：0

期号：6

起止页码：1145-1154

中文期刊名：园艺学报

外文期刊名：Acta Horticulturae Sinica

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：林业公益性行业科研专项经费项目(201504707);国家自然科学基金项目(31470697);中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2017MB007)

语种：中文

中文关键词：金花茶;杂交品种;类黄酮;花色;超高效液相色谱–四极杆–飞行时间质谱

外文关键词：Camellia nitidissima;hybrid cultivar;flavonoids;flower color;ultra performance liquid chromatography quadrupole-time-of-flight mass spectrometry

分类号：S685.14

摘要：以金花茶(Camellianitidissima,深黄色)和山茶(C.japonica,白色)及其杂交品种(淡黄色)为试验材料,按照CIE L~*a~*b~*表色系法测量其花色,利用超高效液相色谱—四极杆—飞行时间质谱(UPLC-Q-TOF-MS)联用技术定性定量分析其类黄酮成分与含量,运用多元线性回归方法研究其花色与类黄酮成分之间的关系。在金花茶和白色山茶及其3个杂交品种中共检测到10种类黄酮成分,其中天竺葵素–3–O–葡萄糖苷(Pg3G)、木犀草素–7–O–芸香糖苷(Lu7R)、圣草素(Er)和染料木苷(Gin)为金花茶中首次发现,而在白色山茶品种‘银白查理斯’和‘白凤’中未检测到Pg3G、Er和Gin,Lu7R含量极低。金花茶中主要成分为槲皮素–3–O–葡萄糖苷(Qu3G)、槲皮素–3–O–芸香糖苷(Qu3R)、槲皮素–7–O–葡萄糖苷(Qu7G)、山萘酚–3–O–葡萄糖苷(Km3G)、Pg3G和Lu7R。杂交品种中木犀草素(Lu)、山萘酚(Km)和Er含量高于双亲,其余成分及类黄酮总量介于双亲之间。Qu3G和Qu3R是决定金花茶及其与白色山茶杂交的品种花瓣呈现黄色的主要成分,Qu3G和Qu3R含量的增加导致花色显著变黄;Qu3G含量的积累显著增加了花色鲜艳程度,Pg3G影响色调。
The flower colors,flavonoid components and contents in dark yellow Camellia nitidissima,white C.japonica and their pale yellow hybrid cultivars were studied.The flower colors were measured by CIE L~*a~*b~*scale,and flavonoids were qualitatively and quantitatively analyzed by ultra-performance liquid chromatography quadrupole-time-of-flight mass spectrometry.The relationship between flower colors and flavonoid components was explored by analyzed by multiple liner regression analyses.Ten flavonoids were detected in C.nitidissima,white C.japonica and their three hybrid cultivars.The flavonoids including pelargonium-3-O-glucoside(Pg3G),luteolin-7-O-rutinoside(Lu7 R),eriodictyol(Er)and genistin(Gin)were identified in C.nitidissima for the first time.Pg3G,Er and Gin were not determined in white C.japonica‘Yinbai Chalis’and‘Baifeng’,and the contents of Lu7 R were extremely low.The main components of C.nitidissima were quercetin-3-O-glucoside(Qu3G),quercetin-3-O-rutinoside(Qu3R),quercetin-7-O-glucoside(Qu7 G),kaempferol-3-O-glucoside(Km3G),Pg3G and Lu7 R.The contents of luteolin(Lu),kaempferol(Km)and Er in hybrid cultivars surpassed their parents,and those of the rest and total flavonoids were between their parents.Qu3G and Qu3R were the important components which decided the yellow color of C.nitidissima and its hybrid cultivars,and the increase of their contents caused the flower color to remarkably turn yellow.The accumulation of Qu3G significantly enhanced the brilliance of flower color,and Pg3G affected the color tone.