文献类型：期刊文献

英文题名：Arabidopsis cysteine-rich receptor-like kinase 45 functions in the responses to abscisic acid and abiotic stresses

作者：Zhang, Xiujuan[1] Yang, Guanyu[1] Shi, Rui[1] Han, Xiaomin[1] Qi, Liwang[2] Wang, Ruigang[1] Xiong, Liming[1,3] Li, Guojing[1]

第一作者：Zhang, Xiujuan

通信作者：Li, GJ[1]

机构：[1]Inner Mongolia Agr Univ, Coll Life Sci, Hohhot 010018, Inner Mongolia, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[3]4700 King Abdullah Univ Sci & Technol KAUST, Div Chem & Life Sci & Engn, Plant Stress Genom Res Ctr, Thuwal 239556900, Saudi Arabia

年份：2013

卷号：67

起止页码：189-198

外文期刊名：PLANT PHYSIOLOGY AND BIOCHEMISTRY

收录：;Scopus(收录号：2-s2.0-84876344016);WOS:【SCI-EXPANDED(收录号:WOS:000319711700023)】；

基金：We thank Dr. Shuqun Zhang for critical reading of the manuscript, and Kathleen M. Buckley, M.S. for editing assistance. This work was supported by the grants from National Natural Science Foundation and Ministry of Education of China (grant nos. 30860029 and NCET-11-1020 to R.W.), and from Ministry of Science and Technology of China and Inner Mongolia Agricultural University (grant nos. 2011AA100203 and NDPYTD2010-3 to G.L.).

语种：英文

外文关键词：Abscisic acid; Arabidopsis thaliana; CRK45; Drought; Early seedling establishment; Germination; Salt

摘要：The phytohormone abscisic acid (ABA) regulates seed germination, plant growth and development, and response to abiotic stresses such as drought and salt stresses. Receptor-like kinases are well known signaling components that mediate plant responses to developmental and environmental stimuli. Here, we characterized the biological function of an ABA and stress-inducible cysteine-rich receptor-like protein kinase, CRK45, in ABA signaling in Arabidopsis thaliana. The crk45 mutant was less sensitive to ABA than the wild type during seed germination and early seedling development, whereas CRK45 overexpression plants were more sensitive to ABA compared to the wild type. Furthermore, over-expression of CRK45 led to hypersensitivity to salt and glucose inhibition of seed germination, whereas the crk45 mutant showed the opposite phenotypes. In addition, CRK45 overexpression plants had enhanced tolerance to drought. Gene expression analyses revealed that the expression of representative stress-responsive genes was significantly enhanced in CRK45 overexpression plants in response to salt stress. ABA biosynthetic genes such as NCED3,(2) NCED5,(3) ABA2,(4) and AAO3(5) were also constitutively elevated in the CRK45 overexpression plants. We concluded that CRK45 plays an important role in ABA signaling that regulates Arabidopsis seeds germination, early seedling development and abiotic stresses response, by positively regulating ABA responses in these processes. (C) 2013 Elsevier Masson SAS. All rights reserved.