文献类型：期刊文献

英文题名：Transcriptome Characterization of Gnetum parvifolium Reveals Candidate Genes Involved in Important Secondary Metabolic Pathways of Flavonoids and Stilbenoids

作者：Deng, Nan[1] Chang, Ermei[1] Li, Minghe[2] Ji, Jing[1] Yao, Xiamei[1] Banish, Igor V.[3] Liu, Jianfeng[1] Ma, Jing[1] Chen, Lanzhen[4,5] Jiang, Zeping[1] Shi, Shengqing[1]

第一作者：Deng, Nan

通信作者：Jiang, ZP[1];Shi, SQ[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Fujian Agr & Forestry Univ, Coll Landscape Architecture, Fuzhou, Peoples R China;[3]Acad Sci Czech Republ, Inst Bot, Dept Genet Ecol, Prague, Czech Republic;[4]Chinese Acad Agr Sci, Inst Apicultural Res, Beijing 100193, Peoples R China;[5]Minist Agr, Risk Assessment Lab Bee Prod Qual & Safety, Beijing, Peoples R China

年份：2016

卷号：7

期号：MAR2016

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000371289100001)】；

基金：This work was supported by the Special Fund for State Key Laboratory of Tree Genetics and Breeding (TGB2013012), 948 project (2012-4-43), and Fund of National Non-profit Research Institutions of CAP (RIF2013-12). We greatly appreciate Prof. Yongzhen Pang for kind suggestions and careful revision for this manuscript, and Ms. Xiaojia Su for her great help during the determination of secondary metabolites in the lab of Prof Pang. We also thank Edanz Editing for copyediting the manuscript.

语种：英文

外文关键词：Gnetophyta; Gnetum; evolution; natural bioactive compounds; gene expression

摘要：Gnetum is a small, unique group of Gnetophyta with a controversial phylogenetic position. Gnetum parvifolium is an important Chinese traditional medicinal plant, which is rich in bioactive compounds such as flavonoids and stilbenoids. These compounds provide significant medicinal effects, mostly as antioxidant, anticancer, and antibacterial agents. However, the mechanisms involved in the biosynthesis and regulation of these compounds in G. parvifolium are still unknown. In this study, we found that flavonoids and stilbene compounds accumulated at different levels in various tissues of G. parvifolium. We further obtained and analyzed massive sequence information from pooled samples of G. parvifolium by transcriptome sequencing, which generated 94,816 unigenes with an average length of 724 bp. Functional annotation of all these unigenes revealed that many of them were associated with several important secondary metabolism pathways including flavonoids and stilbenoids. In particular, several candidate unigenes (PAL-, C4H-, 4CL-, and STS-like genes) involved in stilbenoids biosynthesis were highly expressed in leaves and mature fruits. Furthermore, high temperature and UV-C strongly induced the expression of these genes and enhanced stilbene production (i.e., resveratrol and piceatannol) in leaves of young seedlings. Our present transcriptomic and biochemical data on secondary metabolites in G. parvifolium should encourage further investigation on evolution, ecology, functional genomics, and breeding of this plant with strong pharmaceutical potential.