文献类型：期刊文献

中文题名：3种桉树组培苗不定根发生发育过程的解剖学观察

英文题名：Research of Anatomy Observation on Adventitious Rooting Genesis and Development of Three Rooting Culture Seedlings of Eucalyptus

作者：韩超[1] 徐晓立[2]

第一作者：韩超

机构：[1]中国林业科学研究院热带林业研究所;[2]华南农业大学测试中心

年份：2016

卷号：36

期号：8

起止页码：1594-1599

中文期刊名：西北植物学报

外文期刊名：Acta Botanica Boreali-Occidentalia Sinica

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：中央级公益性科研院所基本科研业务费专项(CAFYBB2014QA011)

语种：中文

中文关键词：桉树;根系;石蜡切片;不定根发育

外文关键词：Eucalyptus; root; paraffin section; adventitious roots development

分类号：Q944.54;S791.248.05

摘要：以巨尾桉‘GL9’、尾巨桉‘DH32-29’和尾边桉‘XF35’3种桉树无性系组培生根苗为材料,采用常规石蜡切片技术,对石蜡切片制作过程中的固定环节进行了优化,观察了桉树不定根的发育过程。结果表明:(1)采用FAA固定液固定材料,可获得染色清晰,组织完整的桉树根系切片。(2)‘GL9’和‘DH32-29’在生根诱导8d后生出不定根,生根类型为皮部生根;‘XF35’在生根诱导12d后生出不定根,生根类型为愈伤组织生根;‘GL9’不定根的根尖和根基处均有细胞旺盛分裂,‘DH32-29’不定根只在根尖有细胞旺盛分裂,‘XF35’不定根则只在其根基处有大量旺盛分裂的细胞。
This paper optimized the fixing procedure of the routine paraffin section technology and observed the process of adventitious rooting development of three culture rooting seedlings of Eucalyptus clones‘DH32-29＇,‘GL9＇and‘XF35＇.The results showed that：（1）FAA fixing solution was better with clear staining and tissue integrity root slice on Eucalyptus.（2）Adventitious roots of‘DH32-29＇and‘GL9＇originated after 8days being induced and belongs to cortices rooting type.Adventitious roots of‘XF35＇originated after 12 days being inducted and belongs to callus rooting type.Active-division cells are in root tip and root foundation of‘GL9＇adventitious root.Active-division cells are only in root tip of‘DH32-29＇adventitious root.Active-division cells are only in root foundation of‘XF35＇adventitious root.