文献类型：期刊文献

中文题名：金花茶花朵和叶片UPLC-QTOF-MS分析

英文题名：UPLC-QTOF-MS Analysis of Flowers and Leaves of Camellia nitidissima

作者：李辛雷[1] 王佳童[1,2] 孙振元[2] 王洁[1] 殷恒福[1] 范正琪[1] 李纪元[1]

第一作者：李辛雷

机构：[1]中国林业科学研究院亚热带林业研究所;[2]林木遗传育种国家重点实验室中国林业科学研究院林业研究所

年份：2018

卷号：31

期号：6

起止页码：83-88

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：林业公益性行业科研专项项目(201504707);国家自然科学基金项目(31470697);中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2017MB007);中央级公益性科研院所基本科研业务费专项资金项目(RISF61250)

语种：中文

中文关键词：金花茶;花朵;叶片;花青苷;类黄酮;超高效液相色谱-飞行时间质谱联用(UPLC-QTOF-MS)

外文关键词：Camellia nitidissima;flowers;leaves;anthocyanins;flavonoids;ultra-performance liquid chromatography quadrupole time of flight mass spectrometry(UPLC-QTOF-MS)

分类号：S718.43

摘要：[目的]鉴定分析金花茶花朵和叶片中主要化学成分、含量及其变化特征,为金花茶资源的进一步开发利用提供科学依据。[方法]利用超高效液相色谱-飞行时间质谱联用(UPLC-QTOF-MS)技术定性定量分析金花茶花瓣、雄蕊、老叶和新叶中花青苷、类黄酮及儿茶素类成分与含量。[结果]花青苷天竺葵素-3-O-葡萄糖苷和矢车菊素-3-O-葡萄糖苷均为金花茶中首次发现,其中,矢车菊素-3-O-葡萄糖苷仅存在于紫红色新叶中。类黄酮木犀草素-7-O-芸香糖苷和染料木苷为金花茶中首次发现,槲皮素-3-O-葡萄糖苷、槲皮素-7-O-葡萄糖苷、槲皮素-3-O-芸香糖苷和山柰酚-3-O-葡萄糖苷为金花茶叶片中首次发现。金花茶花瓣与雄蕊中花青苷相差不大,但却低于叶片尤其新叶;花朵中儿茶素类远高于叶片尤其新叶。金花茶花瓣和雄蕊中总黄酮及槲皮素-3-O-葡萄糖苷、槲皮素-3-O-芸香糖苷和山柰酚-3-O-葡萄糖苷均相差不大,但却远高于叶片。金花茶新叶中主要类黄酮成分木犀草素-7-O-芸香糖苷及总类黄酮明显高于老叶。[结论]金花茶中共鉴定出2种花青苷、6种类黄酮和2种儿茶素;槲皮素-3-O-葡萄糖苷等类黄酮是金花茶花朵呈现黄色的主要原因,矢车菊素-3-O-葡萄糖苷是金花茶新叶呈现紫红色的主要原因。
[Objective]To identify and analyze the main chemical components,their contents and change in the flowers and leaves of Camellia nitidissima,and to provide the basis for further exploitation and utilization of C.nitidissima.[Method]The components and contents of anthocyanins,flavonoids and catechins in the flowers and leaves of C.nitidissima were qualitatively and quantitatively analyzed by ultra-performance liquid chromatography quadrupole time of flight mass spectrometry.[Result]Cyanidin-3-O-glucoside and pelargonium-3-O-glucoside were identified in C.nitidissima for the first time,and cyanidin-3-O-glucoside was only found in the new purple leaves.Luteolin-7-O-rutinoside and genistin were identified in C.nitidissima for the first time,and the quercetin-3-O-glucoside,quercetin-7-O-glucoside,quercetin-3-O-rutinoside and kaempferol-3-O-glucoside were found in leaves of C.nitidissima for the first time.The contents of anthocyanins between petals and stamens had no obvious difference,but were lower than that in leaves,especially new leaves.Instead,the contents of catechins in flowers were well higher than that in leaves,especially new leaves.The contents of total flavonoids,quercetin-3-O-glucoside,quercetin-3-O-rutinoside and kaempferol-3-O-glucoside between petals and stamens had no obvious differences,but were far higher than that in leaves.The contents of total flavonoids and luteolin-7-O-rutinoside which was the main flavonoids in new leaves were significantly higher than that in old leaves.[Conclusion]Two anthocyanins,six flavonoids and two catechins are identified in C.nitidissima.The quercetin-3-O-glucoside and other flavonoids are the main cause of the yellow color of flowers,and the cyanidin-3-o-glucoside is the main cause of the purple color of new leaves of C.nitidissima.