文献类型：期刊文献

中文题名：昆虫RNAi:二十年回顾与展望

英文题名：RNA interference in insects:A twenty-year review and future perspectives

作者：吕子豪[1] 马海宾[1] 张文庆[2]

第一作者：吕子豪

机构：[1]中国林业科学研究院热带林业研究所,广州510520;[2]中山大学生命科学学院昆虫学研究所,广州510275

年份：2025

卷号：62

期号：5

起止页码：1299-1313

中文期刊名：应用昆虫学报

外文期刊名：Chinese Journal of Applied Entomology

收录：;北大核心:【北大核心2023】；

基金：广东省重点研发计划项目(2020B020214001);国家自然科学基金(32372519)。

语种：中文

中文关键词：昆虫;RNA干扰;基因功能;RNA生物农药;害虫控制;脱靶效应

外文关键词：insects;RNA interference;gene function;RNAi-based biopesticides;pest control;off-target effects

分类号：Q963

摘要：自21世纪初我国系统开展昆虫RNAi研究以来,该领域取得了显著进展,科研论文的发表数量与质量现均已达到国际先进水平。本文系统回顾了近二十年来RNAi技术对中国昆虫学研究的重大推动作用。借助RNAi技术,我国学者在昆虫基因功能解析方面不断深入,揭示了昆虫翅型分化等重要生物过程的分子机制。同时,研究者们逐步探索了RNAi技术在田间应用的潜力,在靶标基因筛选、dsRNA递送技术、RNAi脱靶风险预测及产业化应用等方面积累了大量成果。目前,我国在政策支持与技术储备方面已具备良好基础,加快推进RNA生物农药的研发与应用,将显著提升我国农林产业的科技竞争力,为推动农林业绿色可持续发展提供有力支撑。
Remarkable progress has been in research on insect RNAi in China since research began in this field at the beginning of the 21st century.Indeed,China now leads the world in both the quantity and quality of scientific publications in this field.This review comprehensively summarizes the significant contributions of RNAi technology to entomological research in China over the past two decades.Using RNAi,Chinese researchers have deepened understanding of insect gene functions and uncovered a range of novel molecular mechanisms underlying various biological processes,such as wing type differentiation.Meanwhile,considerable advances have been made in exploring the potential of RNAi for field applications,including the identification of target genes,development of dsRNA delivery systems,prediction of off-target risks,and industrial implementation.With favorable policy support and a technological foundation already in place,accelerating the development and application of RNA biopesticides will significantly enhance the competitiveness of China’s agriculture and forestry sectors,and provide substantial support for environmentally-friendly,sustainable agricultural development.