文献类型：期刊文献

英文题名：Understanding saline and osmotic tolerance of Populus euphratica suspended cells

作者：Gu, Ruisheng[1] Liu, Qiunlu[2] Pei, Dong[3,4] Jiang, Xiangning[2]

第一作者：Gu, Ruisheng

通信作者：Pei, D.

机构：[1] Inst. of Appl. Sci. and Technology, Lisbon University, Lisbon, 1749-016, Portugal; [2] Center of Forest Biology, Beijing Forest University, Beijing, 100083, China; [3] Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China; [4] Institute of Forestry, The Chinese Academy of Forestry, Yuheyuan Hou, Beijing 100091, China

年份：2004

卷号：78

期号：3

起止页码：261-265

外文期刊名：Plant Cell, Tissue and Organ Culture

收录：EI(收录号：2004338312537);Scopus(收录号：2-s2.0-3442885739)

语种：英文

外文关键词：Growth kinetics - Morphology - Osmosis - Plants (botany) - Salinity measurement - Sodium chloride

摘要：Tolerance of Populus euphratica suspended cells to ionic and osmotic stresses implemented respectively by NaCl and PEG (6000) was characterized by monitoring cell growth, morphological features, ion compartmentation and polypeptide patterns. The cells grew and proliferated when submitted to stresses of 137 mM NaCl or 250 g l-1 PEG, and survived at 308 mM of NaCl, showing tolerance to saline and particularly osmotic stress. They were resistant to plasmolysis and had dense cytoplasms, large nuclei and nucleoli, and evident cytoplasmic strands under high saline and osmotic stress. The sequestration of Cl- into the vacuoles was observed in the cells stressed with 137 and 223 mM NaCl. The cellular protein profile was modified by high salt and osmotic stress and showed 28 kDa polypeptides up-regulated by both NaCl and PEG, and 66 and 25 kDa polypeptides up-regulated only by high NaCl stress. The salt tolerance of P. euphratica cells might be related to their capacity of adapting to higher osmotic stress by maintaining cell integrity, sequestrating Cl - into vacuoles and modulating polypeptides that reflect cellular metabolic adaptations.