文献类型：期刊文献

英文题名：Novel R2R3-MYB Transcription Factor LiMYB75 Enhances Leaf Callus Regeneration Efficiency in Lagerstroemia indica

作者：Jiang, Shengji[1] Lv, Fenni[1] Gao, Lulu[1] Gu, Jiaojiao[1,2] Yang, Rutong[1] Li, Sumei[1] Li, Ya[1] Li, Shaofeng[3] Wang, Peng[1,2]

第一作者：Jiang, Shengji

通信作者：Wang, P[1];Wang, P[2];Li, SF[3]

机构：[1]Jiangsu Prov & Chinese Acad Sci, Inst Bot, Jiangsu Key Lab Res & Utilizat Plant Resources, Nanjing 210014, Peoples R China;[2]Nanjing Forestry Univ, Coll Forestry, Nanjing 210037, Peoples R China;[3]Chinese Acad Forestry, Expt Ctr Forestry North China, State Key Lab Tree Genet & Breeding, Natl Permanent Sci Res Base Warm Temperate Zone Fo, Beijing 100091, Peoples R China

年份：2023

卷号：14

期号：3

外文期刊名：FORESTS

收录：;EI(收录号：20231513863445);Scopus(收录号：2-s2.0-85151712554);WOS:【SCI-EXPANDED(收录号:WOS:000958479500001)】；

基金：This work was funded by the Chinese Academy of Forestry-Special funds for basic scientific research service expenses of the central level public welfare research institutes (Grant No. CAFYBB2020QD001), the Forestry Science and Technology Promotion Demonstration Fund Project of Chinese Central Finance (Grant No. Su [2021]TG07), and the Opening Foundation of Jiangsu Key Laboratory for the Research and Utilization of Plant Resources (Grant No. JSPKLB201929 and JSPKLB202021).

语种：英文

外文关键词：whole-leaf explants; callus induction; genetic transformation; plant hormone; regeneration ability

摘要：Lagerstroemia indica is an important woody ornamental plant worldwide. However, the application of many technologies, such as transgenic breeding and genome editing, has been severely hampered due to the lack of efficient calli induction and regeneration technology. Here, we discussed a reliable and efficient calli induction and regeneration protocol using whole-leaf explants. This protocol's effectiveness for the calli induction and regeneration systems in crape myrtle were up to 70.33% and 44.33%, respectively. Next, an efficient and stable Agrobacterium-mediated genetic transformation system was created from leaf calli, and the green fluorescent protein (GFP) was able to detect up to 90% of its positive frequency. Meanwhile, two positive lines' transfer DNA insertion sites and directions were identified using whole genome sequencing. LiMYB75, a novel R2R3-MYB transcription factor, was identified and transferred to the L. indica genome to enhance the leaf calli regeneration frequency. Surprisingly, overexpressing LiMYB75 increased the frequency of calli regeneration in the leaf by 1.27 times and the number of regenerated plantlets per callus by 4.00 times compared to the wild type, by regulating the expression levels of genes involved in callus formation, such as SHOOT MERISTEMLESS (STM). Overall, our findings revealed a simple, reliable, and highly efficient transformation approach and identified the desirable candidate gene LiMYB75, which improves L. indica's calli regeneration efficiency.