文献类型：期刊文献

英文题名：Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application

作者：Yue, Jin-Jun[1] Yuan, Jin-Ling[1] Wu, Fu-Hui[2] Yuan, Yu-Hsuan[2] Cheng, Qiao-Wei[2] Hsu, Chen-Tran[2] Lin, Choun-Sea[2]

第一作者：岳晋军

通信作者：Lin, CS[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou, Peoples R China;[2]Acad Sin, Agr Biotechnol Res Ctr, Taipei, Taiwan

年份：2021

卷号：3

外文期刊名：FRONTIERS IN GENOME EDITING

收录：Scopus(收录号：2-s2.0-85122827647);WOS:【ESCI(收录号:WOS:001006596400001)】；

语种：英文

外文关键词：RNP; transient transfection; DNA-free; CRISPR; cas (clustered regularly interspaced short palindromic repeats); protoplasts

摘要：In the clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas) system, protoplasts are not only useful for rapidly validating the mutagenesis efficiency of various RNA-guided endonucleases, promoters, sgRNA designs, or Cas proteins, but can also be a platform for DNA-free gene editing. To date, the latter approach has been applied to numerous crops, particularly those with complex genomes, a long juvenile period, a tendency for heterosis, and/or self-incompatibility. Protoplast regeneration is thus a key step in DNA-free gene editing. In this report, we review the history and some future prospects for protoplast technology, including protoplast transfection, transformation, fusion, regeneration, and current protoplast applications in CRISPR/Cas-based breeding.