文献类型：期刊文献

中文题名：TDZ诱导中间锦鸡儿植株再生

英文题名：Inducing Regeneration of Caragana intermedia by TDZ

作者：胡钠梅[1,2] 韩素英[2] 梁国鲁[1] 齐力旺[2]

第一作者：胡钠梅

机构：[1]西南大学园艺园林学院;[2]中国林科院林业研究所细胞生物学实验室

年份：2009

期号：8

起止页码：204-205

中文期刊名：北方园艺

外文期刊名：Northern Horticulture

收录：北大核心:【北大核心2008】；

基金：国家"十一五"科技支撑资助项目(2006BAD01A16);国家高科技研究发展计划资助项目(863计划)(2007AA10Z182);国家高科技研究发展计划(863计划)资助项目(2007AA100105)

语种：中文

中文关键词：中间锦鸡儿;TDZ;快繁体系

外文关键词：Caraganaintrmedia TDZ Regeneration

分类号：S565.9

摘要：以中间锦鸡儿种子无菌萌发得茎段为外植体,不同激素组合诱导丛生芽,得出最佳丛生芽诱导培养基为MS+TDZ 0.1 mg/L+NAA 0.02 mg/L+糖30.0 g/L+琼脂9 g/L,芽诱导率达220%。诱导得到的丛生芽直接生根困难,需过渡培养。在培养基MS+BA 0.2 mg/L+NAA 0.4 mg/L+糖30.0 g/L+琼脂9 g/L上过渡培养三代以上后转入生根培养基1/2MS+IAA 0.5 mg/L+糖30.0 g/L+琼脂9 g/L生根,长出的根粗壮,且须根数多,生根率在85%以上。生根培养后在草炭土∶珍珠岩=4∶1的基质上移栽成活率达到80%。
An effective protocol for plant generation was developed from young stem segments of C. intermedia aseptic seeds. The results showed that the most suitable medium for inducing adventitious buds was MS4-TDZ 0. 1 mg/L＋ NAA 0. 02 mg/L＋sugar 30.0 g/L＋agar 9 g/L, and the adventitious buds induction is up to 220% after 46 days cultivation. Regenerated buds grew strongly on MS＋BA 0.2 mg/L＋NAA 0. 4 mg/＋bsugar 30.0 g/＋agar 9 g/L. The rooting rate was 85% in the rooting medium 1/2MS＋IAA 0. 5 mg/L＋sugar 30.0 g/L＋agar 9 g/L after 40 days cultivation. The suitable proportion of transplanting medium （mixed turfy soil plus perlite）was 4 ： 1, and in which the transplanting survival rate of plantlets was 80%.