文献类型：期刊文献

英文题名：Proline-rich protein gene PdPRP regulates secondary wall formation in poplar

作者：Li, Shaofeng[1] Zhang, Yaoxiang[1] Ding, Changjun[2] Gao, Xu[1] Wang, Ran[1] Mo, Wenjuan[1] Lv, Fuling[1] Wang, Shaoli[1] Liu, Liang[1] Tang, Zhimin[1] Tian, Hua[1] Zhan, Jianhui[3] Zhang, Bingyu[2] Huang, Qinjun[2] Lu, Mengzhu[2] Wuyun, Ta-na[4] Hu, Zanmin[5] Xia, Yongxiu[1] Su, Xiaohua[2]

通信作者：Xia, YX[1];Su, XH[2]

机构：[1]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 100023, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]North Carolina Cent Univ, Biomfg Res Inst & Technol Enterprise, Dept Pharmaceut Sci, Durham, NC 27707 USA;[4]Chinese Acad Forestry, Nontimber Forest Res & Dev Ctr, Zhengshou 450003, Peoples R China;[5]Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

年份：2019

卷号：233

起止页码：58-72

外文期刊名：JOURNAL OF PLANT PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000455473000007)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 31770705), the Open Fund of State Key Laboratory of Tree Genetics and Breeding (Chinese Academy of Forestry) (Grant No. TGB2013002), and the National Key Program on Transgenic Research (Grant No. 2018ZX08020002).

语种：英文

外文关键词：Molecular mechanisms; Vascular growth; PdPRP; Populus deltoids; Secondary wall formation

摘要：Proline-rich protein (PRP) is a plant cell wall associated protein. Its distinct patterns of regulation and localization studied in a number of plants indicate that it may play important roles in growth and development. However, the mechanism of how these genes control secondary cell wall development in tree species is largely unknown. Here, we report that a Populus deltoides (Marsh.) proline-rich protein gene PdPRP was preferentially expressed in immature/mature phloem and immature xylem in P. deltoides. PdPRP overexpression increased poplar plant height and diameter as well as the radial width of the phloem and xylem regions, facilitated secondary wall deposition, and induced expression of genes related to microfibril angle (MFA) and secondary wall biosynthesis. Downregulation of PdPRP retarded poplar growth, decreased the radial width of the secondary phloem and secondary xylem regions, reduced secondary wall thickening in fibers and vessels, and decreased the expression of genes related to MFA and secondary wall biosynthesis. These results suggest that PdPRP might positively regulate secondary cell wall formation by promoting secondary wall thickening and expansion in poplar. PdPRP-overexpressing poplar had a lower MFA, indicating that PdPRP may be useful for improving wood stiffness and properties in plants. Together, our results demonstrate that PdPRP is a proline-rich protein associated with cell wall development, playing a critical role in regulating secondary cell wall formation in poplar.