文献类型：期刊文献

英文题名：Establishment of an in vitro plant regeneration protocol for Casuarina cunninghamiana Miq. via indirect organogenesis

作者：Jiang, Qingbin[1] Zhang, Yong[1] Zhong, Chonglu[1] Zeng, Bingshan[1] Bogusz, Didier[2] Franche, Claudine[2]

第一作者：Jiang, Qingbin

通信作者：Zhong, CL[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China;[2]IRD, Equipe Rhizogenese, UMR DIADE, F-34394 Montpellier 5, France

年份：2012

卷号：43

期号：2

起止页码：143-154

外文期刊名：NEW FORESTS

收录：;EI(收录号：20120614742824);Scopus(收录号：2-s2.0-84856501992);WOS:【SCI-EXPANDED(收录号:WOS:000300081900002)】；

基金：The authors gratefully acknowledge Drs Simin Gan and Jie Zeng of the Research Institute of Tropical Forestry, Mr Vitoon Luangviriyasaeng of Royal forest Department (Thailand) for their technical assistance. Drs David Bush and John Doran of CSIRO Australia and Dr Daphne Goodfellow of IRD are thanked for their comments on early draft. The authors are grateful to the funding support from the Chinese National Key Project (No. 2009BADB2B0303, No. 2009BADB2B0101, No. 2006BAD01A1605), the Guangdong Key Project (No. 2004B20801008), Guangdong FNST project No. 06024658, SFA Extension Project No. 2010-9, and IRD BESCD project No. 17334.

语种：英文

外文关键词：Casuarina cunninghamiana; Micropropagation; Indirect organogenesis; Plant regeneration

摘要：An in vitro plant regeneration protocol via indirect organogenesis from morphogenetic callus was established for Casuarina cunninghamiana Miq. Effects of plant growth regulator NAA (naphthaleneacetic acid) and BAP (6-benzylaminopurine), sucrose and AgNO3 on callus induction, adventitious bud differentiation and shoot development were examined. Explants used were epicotyl fragments from 45-day-old seedlings. The largest callus (4.29 mm in diameter) was obtained after 1 month on a basic culture medium consisting of Murashige and Skoog A1/2 macro- and full strength micro- elements, Nitsch and Nitsch vitamins, supplemented with 0.54 mu M NAA, 3.30 mu M BAP, and 30 g L-1 sucrose. The calli were subcultured in the same medium above for 2 months. They were then cultured for another 2 months for adventitious bud differentiation and shoot development. The highest mean adventitious bud differentiation, number of shoots formed per callus and number of shoots a parts per thousand yen2 cm long per callus (47.50%, 27.38 and 4.75, respectively) were achieved on the above medium modified with NAA at 0.27 mu M and supplemented with AgNO3 1 mg L-1. Shoots were successfully rooted without plant growth regulator and the rooted plantlets survived and grew normally. This protocol for in vitro plant regeneration provides a tool not only for vegetative propagation but also for plant genetic transformation and gene function studies of C. cunninghamiana.