文献类型：期刊文献

中文题名：灰木莲种质资源遗传多样性的SSR分析

英文题名：Genetic Diversity Analysis of Manglietia conifera Dandy Germplasm by SSR Markers

作者：文珊娜[1] 仲崇禄[1] 姜清彬[1] 张勇[1] 陈珍[1] 陈羽[1]

第一作者：文珊娜

机构：[1]中国林业科学研究院热带林业研究所

年份：2017

卷号：15

期号：9

起止页码：3788-3797

中文期刊名：分子植物育种

外文期刊名：Molecular Plant Breeding

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：林业公益性行业科研专项项目(201304113-01)资助

语种：中文

中文关键词：灰木莲;SSR;遗传多样性;种质资源

外文关键词：Manglietia conifera, SSR, Genetic diversity, Germplasm

分类号：S

摘要：为探索灰木莲种质资源的遗传多样性,利用SSR分子标记技术,对越南灰木莲13个种源的遗传多样性分析。结果表明:8对SSR引物在13个种源中共检测到133个等位基因,平均每个引物扩增16.63个观测等位基因和5.47个有效等位基因,平均观测杂合度、平均期望杂合度、Shannon's信息指数和Nei's遗传多样性指数分别为0.64、0.71、1.86和0.74。各种源内的有效等位基因数平均为5.47,平均观测杂合度、平均期望杂合度分别为0.60和0.42,13个种源的Shannon's信息指数变化范围为1.41~1.80,Nei's遗传多样性指数的变化范围为0.59~0.75,各种源间的遗传多态性水平存在明显差异。种源间的遗传一致度均值为0.834,变化范围为0.655~0.967;遗传距离均值为0.194,变化范围为0.034~0.423。聚类分析表明,13个种源在遗传距离0.16处可聚为4个类群,种源LC5与其他种源间的亲缘关系较远,在分子遗传水平上的分类结果与其材料来源分类的结果并不完全一致,种源间的遗传距离与地理距离不存在显著的相关性。综上,灰木莲种质资源具有丰富的遗传多样性。
In this study, 8 SSR primer pairs with polymorphism were used to assess genetic diversity and relationship of 13 provenances of Manglietia conifera from Vietnam. The results showed that there were 133 polymorphic SSR loci detected by 8 pairs of SSR markers, with a mean of 16.63 and 5.47 per locus, the average observed and expected heterozygosities, Shannon information index and Nei＇s gene diversity were 0.64, 0.71, 1.86 and 0.74, respectively. The average number of effect alleles, observed and expected heterozygosities of 13 provenances were 5.47, 0.60 and 0.42, respectively, Shannon information index ranged from 1.41-1.80, and Nei＇s gene diversity ranged from 0.59-0.75, the difference of genetic polymorphism level among provenances was obvious. The average value of genetic identities among provenances was 0.834, ranged from 0.655-0.967; the average value of genetic distance was 0.194, ranged from 0.034-0.423. According to the UPGMA cluster analysis,the 13 provenances could be obviously divided into four groups when genetic distance was 0.16, the phylogenetic relationships between provenance LC5 and other provenances were relatively distant, the classification results at the molecular genetic level were not completely consistent with the classification of the material source, there were no significant correlation between genetic and geographic distance among the M. conifera provenances. These results suggested that there is a high level of genetic polymorphism among M. conifera germplasms revealed by SSR markers.