文献类型：期刊文献

中文题名：RAPD技术在珍稀濒危植物遗传多样性研究中的应用

英文题名：Application of RAPD Technique in Research of Genetic Diversity in Rare and Endangered Plants

作者：刘建锋[1] 肖文发[1] 冯霞[1]

第一作者：刘建锋

机构：[1]中国林业科学研究院林业研究所

年份：2004

卷号：40

期号：3

起止页码：156-161

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国务院三峡建设委员会办公室"三峡库区陆生动植物监测"项目资助

语种：中文

中文关键词：RAPD技术;珍稀濒危植物;遗传多样性;生物多样性;保护

外文关键词：Rare and endangered plants, Genetic diversity, RAPD, Application

分类号：Q943.2;Q75

摘要：生物多样性是现代生态学研究的核心问题和热点之一 ,遗传多样性则是一切多样性的基础和最重要的成分。现代分子生物技术的发展 ,为生物遗传多样性研究提供了许多新的方法 ,其中RAPD技术凭借其自身独特的优势成为这一领域的重要研究手段。珍稀濒危物种遗传多样性的研究 ,有助于实施最佳的保护策略 ,因此具有重要的研究意义。本文阐述了RAPD技术的原理及特点 ,综述了该技术在珍稀濒危植物遗传多样性方面的应用 ,并展望了该技术在珍稀濒危物种研究和保护中的应用前景。
Biodiversity is one of the key and the popular subjects of the modern ecological research. Genetic diversity is the foundation and the most important element of biodiversity. With the development of molecular bio|technology, more and more methods have been applied in genetic diversity research. The technique of Random Amplified Polymorphic DNA(RAPD) markers, with its particular advantages,has became an important research means in this field. The research of genetic diversity of rare and endangered plants contributes great to their conservation. The paper described the principle and characteristics of RAPD,and reviewed the application of this technique in research of genetic diversity of rare and endangered plants. The prospect on studying and protecting the rare and endangered plants by RAPD was also advised.