文献类型：会议论文

英文题名：Determination of anthraquinone content in lac dye through combined spectrophotometry and HPCE

作者：Zhang Hong[1,2] Fang Guigan[1] Zheng Hua[2] Li Kun[2]

第一作者：Zhang Hong

通信作者：Fang, GG[1]

机构：[1]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Nanjing 210042, Jiangsu, Peoples R China;[2]Chianese Acad Forestry Kunming, Res Inst Resources Insects, Yunnan 650224, Peoples R China

会议论文集：2nd SREE Conference on Chemical Engineering (CCE)

会议日期：DEC 17-18, 2011

会议地点：Macao, PEOPLES R CHINA

语种：英文

外文关键词：spectrophotometer; high performance capillary electrophoresis (HPCE); lac dye; laccaic acid; anthraquinones

年份：2011

摘要：This study is aim to establish the method for determination of anthraquinone content in lac dye with the combined spectrophotometry and HPCE, With the 0.5% Mg(Ac)(2)-CH3OH solution as the colour-developing agent, the carminic acid concentration within the concentration of 5 similar to 50 mu g/mL presents a good linear relationship with the absorbance under the wavelength of 540nm (R=0.9994). Through the test, the results showed that the total anthraquinone content in lac dye was 82.13%, average recovery rate 97.80%, RSD 1.31%. Under 291nm detection wavelength and 25 degrees C column temperature, with pH8.035 60mmol/LNa2HPO4-Na2B4O7 center dot 10H(2)O mixed buffer as background electrolyte and 60cm x 75 mu m uncoated capillary column as separation lanes, separation voltage 20kV, pressure 0.5psi x 10s for sample injection, the relative contents of five components laccaic acids A, B, C, D, E were measured by peak area normalization method; combined with the spectrophotometry, the contents of five anthraquinone components - laccaic acids A, B, C, D, E were determined, respectively 40.42%, 17.66%, 2.54%, 1.51%, 20.00%.