文献类型：期刊文献

中文题名：长期氮添加对水杉人工林根际和非根际土壤碳氮磷计量特征的影响

英文题名：Effects of Long-term Nitrogen Addition on the Carbon,Nitrogen,and Phosphorus Stoichiometry in Rhizosphere and Bulk Soils of Metasequoia glyptostroboides Plantations

作者：余涵[1,2] 吴统贵[1] 苗永朝[1] 陈颂[1] 王高峰[3] 童冉[1]

第一作者：余涵

机构：[1]中国林业科学研究院亚热带林业研究所,杭州311400;[2]南京林业大学南方现代林业协同创新中心,南京210037;[3]美国克莱姆森大学林业与环境保护系,南卡罗来纳州克莱姆森29634‐0317

年份：2026

卷号：47

期号：1

起止页码：565-572

中文期刊名：环境科学

外文期刊名：Environmental Science

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金项目(32201632);浙江省万人计划科技创新领军人才项目(2023R5251)。

语种：中文

中文关键词：氮添加;根际土壤;非根际土壤;碳氮磷计量特征;影响因素

外文关键词：nitrogen addition;rhizosphere soil;bulk soil;carbon,nitrogen,phosphorus stoichiometric characteristics;influencing factors

分类号：X171.1

摘要：土壤碳、氮、磷含量及其化学计量比的变化,对生物地球化学循环和生态系统功能具有重要的调控作用.然而,土壤碳氮磷计量特征对长期氮沉降的响应及其在根际和非根际土壤间的表现有何差异尚不明晰.基于此,在江苏省盐城市东台林场水杉人工林开展为期10 a的野外试验,设置5个施氮梯度:N0[0 kg·(hm^(2)·a)^(-1)]、N56[56 kg·(hm^(2)·a)^(-1)]、N168[168 kg·(hm^(2)·a)^(-1)]、N280[280 kg·(hm^(2)·a)^(-1)]和N336[336 kg·(hm^(2)·a)^(-1)].测定了根际和非根际土壤基本理化性质、养分计量特征、酶活性和微生物生物量等.结果表明:(1)相比于N0,全部氮添加处理下根际和非根际土壤碳氮比总体分别显著降低了29.0%和13.1%,仅N336处理下根际土壤全氮、氮磷比分别显著升高了25.4%和28.4%,土壤有机碳、全磷和碳磷比对氮添加的响应在全部氮添加及各单独处理下均不显著.(2)根际和非根际土壤碳氮磷计量特征对氮添加的响应方向较为一致,但根际土壤的响应强度更大.(3)非根际土壤有机碳、全氮和全磷含量两两间均存在显著正相关关系,而根际土壤仅有机碳和全氮含量存在显著正相关关系,在根际和非根际土壤中有机碳含量与计量比之间均存在显著正相关关系.(4)微生物生物量及酶活性等微生物性质是影响非根际土壤碳氮磷计量特征的主要因素,而pH、硝态氮和有效磷等化学性质对根际土壤碳氮磷计量特征起主要的调控作用.综合来看,根际和非根际土壤碳氮磷计量特征对长期氮添加的响应较为一致,但受到土壤化学和微生物性质的不同程度的调控.研究结果加深了对环境变化背景下根际和非根际土壤碳和养分响应规律及机制的认识,可为今后生物地球化学循环模型的构建提供科学参考.
Changes in soil carbon,nitrogen,and phosphorus contents and their stoichiometric ratios play a crucial regulatory role in biogeochemical cycles and ecosystem functions.However,the response of soil carbon,nitrogen,and phosphorus stoichiometry to long-term nitrogen deposition,particularly the contrasting patterns between rhizosphere and bulk soils,remains insufficiently understood.Based on this,a ten-year field experiment was conducted in Metasequoia glyptostroboides plantations at Dongtai Forest Farm in Yancheng,Jiangsu Province,with five nitrogen addition gradients:N0[0 kg·(hm^(2)·a)^(?1)],N56[56 kg·(hm^(2)·a)^(?1)],N168[168 kg·(hm^(2)·a)^(?1)],N280[280 kg·(hm^(2)·a)^(?1)],and N336[336 kg·(hm^(2)·a)^(?1)].The basic physical and chemical properties,nutrient stoichiometric characteristics,enzyme activity,and microbial biomass of both rhizosphere and bulk soils were measured.The results indicated:①Compared to that in N0,the overall carbon to nitrogen ratio in rhizosphere and bulk soils under all nitrogen addition treatments decreased significantly by 29.0%and 13.1%,respectively.In the N336 treatment,total nitrogen content and nitrogen to phosphorus ratio in rhizosphere soil significantly increased by 25.4%and 28.4%,respectively.The responses of soil organic carbon and total phosphorus contents and the carbon to phosphorus ratio to nitrogen addition were not significant across all treatments and in each individual treatment.②The response directions of carbon,nitrogen,and phosphorus stoichiometric characteristics in rhizosphere and bulk soils to nitrogen addition were relatively consistent,but the response intensity in rhizosphere soil was greater.③Significant positive correlations were found among the organic carbon,total nitrogen,and total phosphorus contents in bulk soil,whereas only organic carbon and total nitrogen contents showed significant positive correlation in rhizosphere soil.Organic carbon content showed a significant positive correlation with stoichiometric ratios in both rhizosphere and bulk soils.④Microbial biomass and enzyme activity predominantly influenced the carbon,nitrogen,and phosphorus stoichiometric characteristics in bulk soil,whereas chemical properties such as pH,nitrate nitrogen,and available phosphorus contents were the primary regulators of these characteristics in rhizosphere soil.Overall,the responses of carbon,nitrogen,and phosphorus stoichiometric characteristics in rhizosphere and bulk soils to longterm nitrogen addition were relatively consistent but were regulated to varying degrees by soil chemical and microbial properties.This study deepens our understanding of the mechanisms and response patterns of rhizosphere and bulk soil carbon and nutrients to environmental changes,offering valuable scientific insights for advancing biogeochemical cycling models.