文献类型：期刊文献

英文题名：Assessment of Genetic Diversity and Population Genetic Structure of Corylus mandshurica in China Using SSR Markers

作者：Zong, Jian-Wei[1,2] Zhao, Tian-Tian[1] Ma, Qing-Hua[1] Liang, Li-Song[1] Wang, Gui-Xi[1]

第一作者：Zong, Jian-Wei

通信作者：Zhao, TT[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Pingdingshan Univ, Coll Resource & Environm Sci, Pingdingshan, Henan Province, Peoples R China

年份：2015

卷号：10

期号：9

外文期刊名：PLOS ONE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000360965800057)】；

基金：This work was supported by Beijing Municipal Natural Science Foundation (Grant No. 6144030, http://www.bjnsf.org/), the Special Fund from State Forestry Administration in the Public Interest (Grant No. 201304710, http://www.forestry.gov.cn/), and Special Fund for Basic Scientific Research Business of Central Public Research Institutes (Grant No. RIF2013-10, http://rif.caf.ac.cn/).

语种：英文

摘要：Corylus mandshurica, also known as pilose hazelnut, is an economically and ecologically important species in China. In this study, ten polymorphic simple sequence repeat (SSR) markers were applied to evaluate the genetic diversity and population structure of 348 C. mandshurica individuals among 12 populations in China. The SSR markers expressed a relatively high level of genetic diversity (Na = 15.3, Ne = 5.6604, I = 1.8853, Ho = 0.6668, and He = 0.7777). According to the coefficient of genetic differentiation (F-st = 0.1215), genetic variation within the populations (87.85%) were remarkably higher than among populations (12.15%). The average gene flow (Nm = 1.8080) significantly impacts the genetic structure of C. mandshurica populations. The relatively high gene flow (Nm = 1.8080) among wild C. mandshurica may be caused by wind-pollinated flowers, highly nutritious seeds and self-incompatible mating system. The UPGMA (unweighted pair group method of arithmetic averages) dendrogram was divided into two main clusters. Moreover, the results of STRUCTURE analysis suggested that C. mandshurica populations fell into two main clusters. Comparison of the UPGMA dendrogram and the Bayesian STRUCTURE analysis showed general agreement between the population subdivisions and the genetic relationships among populations of C. mandshurica. Group I accessions were located in Northeast China, while Group II accessions were in North China. It is worth noting that a number of genetically similar populations were located in the same geographic region. The results further showed that there was obvious genetic differentiation among populations from Northeast China to North China. Results from the Mantel test showed a weak but still significant positive correlation between Nei's genetic distance and geographic distance (km) among populations (r = 0.419, P = 0.005), suggesting that genetic differentiation in the 12 C. mandshurica populations might be related to geographic distance. These data provide comprehensive information for the development of conservation strategies of these valuable hazelnut resources.