文献类型：期刊文献

英文题名：Characterization of poplar growth-regulating factors and analysis of their function in leaf size control

作者：Wang, Jinnan[1] Zhou, Houjun[1,2] Zhao, Yanqiu[1] Sun, Pengbo[1] Tang, Fang[1,3] Song, Xueqin[1,3] Lu, Meng-Zhu[1,3,4]

通信作者：Song, XQ[1];Lu, MZ[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm Res,Inst Forestry, Beijing 100091, Peoples R China;[2]Ludong Univ, Yantai 264025, Peoples R China;[3]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Peoples R China;[4]Zhejiang Agr & Forestry Univ, Hangzhou 311300, Peoples R China

年份：2020

卷号：20

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;Scopus(收录号：2-s2.0-85095133466);WOS:【SCI-EXPANDED(收录号:WOS:000590051600002)】；

基金：This work was supported by the Basic Research Fund of Research Institute of Forestry, Chinese Academy of Forestry (RIF-2014-08) to X.S, the National Natural Science Foundation of China (31570676) to X.S. The funders did not participate in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文关键词：Growth-regulating factor; Phylogenetic relationship; miR396; Leaf development; Populus

摘要：Background Growth-regulating factors (GRFs) are plant-specific transcription factors that control organ size. Nineteen GRF genes were identified in the Populus trichocarpa genome and one was reported to control leaf size mainly by regulating cell expansion. In this study, we further characterize the roles of the other poplar GRFs in leaf size control in a similar manner. Results The 19 poplar GRF genes were clustered into six groups according to their phylogenetic relationship with Arabidopsis GRFs. Bioinformatic analysis, degradome, and transient transcription assays showed that 18 poplar GRFs were regulated by miR396, with GRF12b the only exception. The functions of PagGRF6b (Pag, Populus alba x P. glandulosa), PagGRF7a, PagGRF12a, and PagGRF12b, representing three different groups, were investigated. The results show that PagGRF6b may have no function on leaf size control, while PagGRF7a functions as a negative regulator of leaf size by regulating cell expansion. By contrast, PagGRF12a and PagGRF12b may function as positive regulators of leaf size control by regulating both cell proliferation and expansion, primarily cell proliferation. Conclusions The diversity of poplar GRFs in leaf size control may facilitate the specific, coordinated regulation of poplar leaf development through fine adjustment of cell proliferation and expansion.