文献类型：期刊文献

英文题名：A chromosome-level genome assembly provides insights into the local adaptation of Tamarix austromongolica in the Yellow River Basin, China

作者：Gong, Shuai[1] Gan, Honghao[1] Chu, Jianmin[1,2] Wang, Zhaoshan[1] Sun, Jia[1]

第一作者：Gong, Shuai

通信作者：Chu, JM[1];Chu, JM[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Coastal Forestry Res Ctr, Natl Forestry & Grassland Adm, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Expt Ctr Desert Forestry, Dengkou 015200, Peoples R China

年份：2024

卷号：31

期号：4

外文期刊名：DNA RESEARCH

收录：;Scopus(收录号：2-s2.0-85200904787);WOS:【SCI-EXPANDED(收录号:WOS:001285472300001)】；

基金：This project was supported by the National Science Foundation (grant no. 32171868) and Fundamental Research Funds of CAF (CAFYBB2020SZ0013).

语种：英文

外文关键词：Tamarix austromongolica; chromosome-level genome assembly; population genetics; local adaptation

摘要：Tamarix austromongolica is endemic to the Yellow River Basin and has adapted to diverse ecological settings in the region, including the arid areas of northwestern China and the saline soil regions of the Yellow River Delta. However, the genetic basis of its local adaptation remains unclear. We report a chromosome-level assembly of the T. austromongolica genome based on PacBio high-fidelity sequencing and Hi-C technology. The 12 pseudochromosomes cover 98.44% of the 1.32 Gb assembly, with a contig N50 of 52.57 Mb and a BUSCO score of 98.2%. The genome comprises 913.6 Mb (68.83%) of repetitive sequences and 22,374 protein-coding genes. Genome evolution analyses suggest that genes under positive selection and significantly expanded gene families have facilitated T. austromongolica's adaptability to diverse environmental factors and high resistance to diseases. Using genotyping-by-sequencing, we conducted population structure and selection analyses of 114 samples from 15 sites. Two genetic groups were identified, and 114 and 289 candidate genes were assigned to the populations of the northwestern and eastern parts of the Yellow River, respectively. Furthermore, we discovered numerous candidate genes associated with high-altitude adaptability and salt tolerance. This research provides valuable genomic resources for the evolutionary study and genetic breeding of tamarisk.