文献类型：期刊文献

中文题名：红翅槭的组织培养及快繁技术

英文题名：Study on Tissue Culture and Rapid Propagation of Acer fabri Hance

作者：唐丽[1] 钟秋平[2] 刘显梅[1] 张利智[1]

第一作者：唐丽

机构：[1]中南林业科技大学林学院;[2]中国林科院亚热带林业实验中心

年份：2010

期号：9

起止页码：136-138

中文期刊名：北方园艺

外文期刊名：Northern Horticulture

收录：北大核心:【北大核心2008】；

基金：中南林业科技大学引进人才资助项目(104-0031)

语种：中文

中文关键词：红翅槭;组织培养;快速繁殖

外文关键词：Acer fabri Hance ; tissue culture ; rapid propagation

分类号：S792.35

摘要：以红翅槭嫩茎和嫩叶为外植体,研究在培养基中添加不同种类、不同浓度的激素后对红翅槭组培快繁的影响。结果表明:嫩茎分化率高,嫩叶分化速率快;接种于不同培养基上的嫩茎、嫩叶在形成愈伤组织和不定芽的时间上有明显的差异,且其长出愈伤组织和芽体的数量也有明显的不同;激素浓度、种类及不同组合对外植体的诱导和分化起着重要的作用,MS+6-BA0.5 mg/L+NAA 0.1 mg/L是嫩茎和嫩叶颗粒状愈伤组织和不定芽分化培养的理想培养基;1/2MS+IAA 0.6 mg/L+NAA 0.1 mg/L是不定芽生根培养的理想培养基;蛭石+珍珠岩(1∶1)是红翅槭试管苗移栽的理想基质,平均成活率为95.3%,并且移栽成活苗生长旺盛。
By Faber Maple Stem cut and new Leaves as seeding, carried on the adult plant regeneration and the rapid propagation research. The results indicated the differentiation rate of stem tip was high, the differentiation rate of leaves was quickly;inoculated in different culture medium on the tip,leaves in the formation of callus and adventitious buds on the time there was significant difference,and the grown callus and shoots the number of precursors were quite different; hormone concentrations,types,and different combinations of external explants induction and differentiation played an important role,MS＋BA 0. 5 mg/L＋NAA 0. 1 mg/L were stem cut and leaves granular callus and adventitious bud differentiation cultivate an ideal medium; 1/2MS＋IAA 0. 6 mg/L＋NAA 0. 1 mg/L adventitious buds were ideal for rooting culture medium;vermiculite and forest humus soil were red-winged maple transplanting in vitro ideal matrix, the average survival rate was 95. 3% and survival of transplanted seedlings grew well.