文献类型：期刊文献

中文题名：真菌诱导子促进桑黄黄酮类物质积累及主要作用机制

英文题名：Fungal Elicitors Promote Flavonoid Biosynthesis in Sanghuangporus vaninii and the Underlying Mechanisms

作者：张宇薇[1,2,3] 莫梦婷[1,2,3] 舒博[1,2,3] 姚佳佳[3] 袁志林[1,3]

第一作者：张宇薇

机构：[1]林木遗传育种全国重点实验室中国林业科学研究院,北京100091;[2]南京林业大学林草学院,南京210037;[3]中国林业科学研究院亚热带林业研究所,杭州311400

年份：2026

卷号：62

期号：3

起止页码：146-160

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：;北大核心:【北大核心2023】；

基金：中央级公益性科研院所基本科研业务费专项资金(CAFYBB2024ZA023-6)。

语种：中文

中文关键词：真菌诱导子;桑黄生物量;黄酮含量;优化条件;桑黄子实体

外文关键词：fungal elicitors;Sanghuangporus vaninii biomass;flavonoid content;optimized conditions;fruiting body

分类号：Q939.96

摘要：【目的】探究不同真菌来源的诱导子、同一真菌不同类型诱导子对桑黄菌丝体和子实体生长和黄酮含量的影响,以全面评估真菌诱导子在桑黄不同生长阶段和培养环境中的效果。【方法】以11种不同土壤真菌制备3种不同类型的真菌诱导子,总计33种真菌诱导子处理桑黄菌丝体,统计分析桑黄生物量、黄酮含量和黄酮产量,并综合3个指标筛选出3种最佳真菌诱导子;通过实时定量PCR初步验证最佳真菌诱导子提升黄酮含量的分子机制;利用响应面法进行最佳真菌诱导子诱导条件的优化,并将最佳真菌诱导子作用于桑黄子实体进行黄酮含量积累的验证;最后将对桑黄黄酮含量提升效果较好的菌株HSL的菌丝体和发酵液的代谢组分进行非靶向代谢组学分析。【结果】3种不同类型的最佳真菌诱导子(分别为NL-19M、NL-19ME和HSLFE)能够促进桑黄生物量、黄酮含量和黄酮产量的积累。3种最佳真菌诱导子通过提高黄酮生物合成途径中关键酶的表达量促进黄酮含量积累。同一真菌诱导子对桑黄生物量、黄酮含量和黄酮产量的优化条件存在差异。NL-19M、NL-19ME、HSLFE处理可提高桑黄子实体的黄酮含量,均高于对照,代谢组分显示真菌诱导子的有效成分可能为脂类物质。【结论】不同真菌诱导子处理能够激活黄酮生物合成途径中不同关键酶的表达以及其存在不同的最佳诱导条件,反映真菌代谢调控的复杂性,揭示出真菌诱导子对黄酮生物合成的调控机制,为真菌诱导子的商业化应用以及林下经济和“以菌养菌、以菌养药”药食用真菌品质提质增效技术体系的建立提供了更完善、系统的理论基础。
【Objective】This study aims to explore the effects of fungal elicitors from different fungi and different types of fungal elicitors from the same fungus on the growth of mycelia&fruiting bodies and flavonoid content,effective medicinal components of Sanghuangporus vaninii,in order to comprehensively evaluate the efficiency of fungal elicitors in different growth stages and culture environments.【Method】Three different types of fungal elicitors were prepared from 11 different soil fungi.A total of 33 different fungal elicitors were used to treat the mycelia of S.vaninii.The biomass,flavonoid content and flavonoid production were statistically analyzed,and three optimal fungal elicitors were screened out based on the three indicators.The realtime quantitative PCR was used to preliminarily verify the molecular mechanism by which three optimal fungal elicitors enhanced flavonoid content.The induction conditions of fungal elicitors were optimized by the response surface method.Three optimal fungal elicitors were applied to the fruiting bodies to verify the accumulation of flavonoid content.Finally,non-targeted metabolomics analysis was conducted on the mycelia and the fermentation broth of strain HSL,which had a better effect in increasing the flavonoid content of S.vaninii.【Result】Three optimal fungal elicitors(NL-19M,NL-19ME and HSLFE)had the best induction effects on the biomass,flavonoid content and flavonoid production of S.vaninii.Moreover,the three fungal elicitors promoted the accumulation of flavonoid content by increasing the expression levels of different key enzymes in the flavonoid biosynthesis pathway.There were differences in the optimization conditions for the biomass,flavonoid content and flavonoid production by the elicitors of the same fungus.The flavonoid contents of the fruiting bodies treated with NL-19M,NL-19ME and HSLFE were all higher than those of the control.The active ingredients of fungal elicitors may be lipids and lipid-like molecules.【Conclusion】Different optimal fungal elicitors treatments can activate the expression of different key enzymes for flavonoid biosynthesis and have different induction conditions,reflecting the complexity of fungal metabolic regulation and revealing the regulatory mechanism of fungal elicitors on the flavonoid biosynthesis.This study provides a more complete and systematic theoretical basis for the commercial application of fungal elicitors and the establishment of a technical system for improving the quality and efficiency of under-forest economy including medicinal and edible fungi.