文献类型：期刊文献

英文题名：Insight into the formation of trumpet and needle-type leaf in Ginkgo biloba L. mutant

作者：Tang, Fang[1,2] Sun, Pengbo[1] Zhang, Qian[3] Zhong, Fengwei[3] Wang, Ying[3] Lu, Mengzhu[1,2,4]

第一作者：唐芳;Tang, Fang

通信作者：Lu, MZ[1];Lu, MZ[2];Wang, Y[3];Lu, MZ[4]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat Natl Forestry & G, Beijing, Peoples R China;[2]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing, Peoples R China;[3]Taishan Acad Forestry Sci, Tai An, Peoples R China;[4]Zhejiang A&F Univ, Sch Forestry & Biotechnol, State Key Lab Subtrop Silviculture, Hangzhou, Peoples R China

年份：2022

卷号：13

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000903001800001)】；

基金：This work was supported by the Special Support Funds for the National Ten-thousand Talents Program of China to ML.

语种：英文

外文关键词：Ginkgo biloba L; leaf shape regulation; anatomical structure; transcriptome analysis; adaxial-abaxial polarity; leaf boundary

摘要：The leaf type of a plant determines its photosynthetic efficiency and adaptation to the environment. The normal leaves of modern Ginkgo biloba, which is known as a "living fossil" in gymnosperm, evolved from needle-like to fan-shaped with obvious dichotomous venation. However, a newly discovered Ginkgo variety "SongZhen" have different leaf types on a tree, including needle-, trumpet-, strip-, and deeply split fan-shaped leaves. In order to explore the mechanism in forming these leaf types, the microscopy of different leaf types and transcriptome analysis of apical buds of branches with normal or abnormal leaves were performed. We found that the normal leaf was in an intact and unfolded fan shape, and the abnormal leaf was basically split into two parts from the petiole, and each exhibited different extent of variation. The needle-type leaves were the extreme, having no obvious palisade and spongy tissues, and the phloem cells were scattered and surrounded by xylem cells, while the trumpet-type leaves with normal vascular bundles curled inward to form a loop from the abaxial to adaxial side. The other type of leaves had the characteristics among needle-type, trumpet-type, or normal leaves. The transcriptome analysis and quantitative PCR showed that the genes related to abaxial domain were highly expressed, while the adaxial domain promoting genes were decreasingly expressed in abnormal-type leaf (ANL) buds and abnormal leaves, which might lead to the obvious abaxialized leaves of "SongZhen." In addition, the low expression of genes related to leaf boundary development in ANL buds indicated that single- or double-needle (trumpet) leaves might also be due to the leaf tissue fusion. This study provides an insight into the mechanism of the development of the abnormal leaves in "SongZhen" and lays a foundation for investigating the molecular mechanism of the leaf development in gymnosperms.