文献类型：期刊文献

英文题名：Effect of karst rocky desertification on soil fungal communities in Southwest China

作者：Wang, P. C.[1] Mo, B. T.[1] Chen, Y.[1] Zeng, Q. F.[1] Wang, L. B.[2]

第一作者：Wang, P. C.

通信作者：Wang, LB[1]|[a00056654ff281abf2d37]王利兵;

机构：[1]Guizhou Inst Prataculture, Guiyang, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Beijing, Peoples R China

年份：2016

卷号：15

期号：3

外文期刊名：GENETICS AND MOLECULAR RESEARCH

收录：;Scopus(收录号：2-s2.0-84979942256);WOS:【SCI-EXPANDED(收录号:WOS:000384881300054)】；

基金：Research supported by the National Natural Science Foundation of China (#31260572), the "973" Project of China (#2014CB138706), and the Guizhou Project of China (Qiankeherenzi #201502).

语种：英文

外文关键词：Fungi; PCR-DGGE; Karst; Rocky desertification; 18S rRNA

摘要：Karst mountainous ecosystems are associated with karst rocky desertification (KRD), which can greatly impact soil structure and function. Despite the importance of soil microbes as a major factor maintaining ecosystem stability, we know little about the effect on soil fungal communities of KRD in karst regions. We investigated this relationship across a gradient of KRD soils from Guizhou, China by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE). Fungal diversity indices (Shannon-Wiener, richness, and evenness) significantly differed (P < 0.05) based on KRD severity, being lowest in moderately affected areas. Cluster analysis showed that the five sites examined clustered into two main groups according to KRD grade (high and low). Moreover, a homology search using sequences recovered from PCR-DGGE bands showed that the dominant fungi in each community varied remarkably, and included Aspergillus, Aphanoascus, Blastomyces, Fusarium, Glomus, Geomyces, Gibberella, Mortierella, Tetracladium, and Tumularia species, and an unclassified group. In conclusion, these findings demonstrate that KRD has a significant impact on soil fungal communities.