文献类型：期刊文献

英文题名：Antifungal activity and mechanism of Litsea cubeba (Lour.) Persoon essential oil against the waxberry spoilage fungi Penicillium oxalicum and its potential application

作者：Yu, Liang[1] Zhou, Yangxia[1] Chen, Yicun[2,3] Wang, Yangdong[2,3] Gu, Qing[1] Song, Dafeng[1,4]

第一作者：Yu, Liang

通信作者：Song, DF[1]

机构：[1]Zhejiang Gongshang Univ, Key Lab Food Microbial Technol Zhejiang Prov, Hangzhou 310018, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[4]Zhejiang Gongshang Univ, Key Lab Food Microbial Technol Zhejiang Prov, Hangzhou, Peoples R China

年份：2024

卷号：411

外文期刊名：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-85178124572);WOS:【SCI-EXPANDED(收录号:WOS:001132099600001)】；

基金：This work was supported by the Science Technology Department of Zhejiang Province, Science and Technology Commissioner: Intensive processing of aquatic products (Dafeng Song) .

语种：英文

外文关键词：Penicillium oxalicum; Litsea cubeba essential oil; Antifungal; Plasma membrane; Food preservation

摘要：Litsea cubeba essential oil (LCEO) is a broad-spectrum bacteriostatic substance produced from the fruit of the Litsea tree that has been used for the treatment of various diseases in China for thousands of years. Here, the antifungal activities of LCEO against 10 different fungi (Naganishia diffluens, Fusarium sacchari, Cladosporium tenuissimum, Fusarium proliferatum, Fusarium verticillioides, Fusarium subglutinans, Mucor racemosus, Penicillium oxalicum, Penicillium chrysogenum, and Aspergillus niger) that cause rot to waxberries were assessed. The chemical components of LCEO and its modes of action against P. oxalicum were investigated. Citral (32.62 %) was characterized as the main component of LCEO by gas chromatography-mass spectrometry. LCEO exhibited excellent antifungal activities against all 10 fungi. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration of LCEO against P. oxalicum were 2.24 and 4.48 g/L, respectively. Furthermore, LCEO (MIC) compromised membrane permeability and integrity, caused leakage of the cell components, and increased production of malondialdehyde and reactive oxygen species. Scanning electron microscopy and transmission electron microscopy indicated that the morphology and ultrastructure of the LCEO-treated hyphal cell membrane and organelles were severely damaged. Meanwhile, LCEO increased the shelf life of waxberries from 1-2 to 5-6 d. LCEO is a potential ecologically friendly alternative to commercial fungicides to inhibit postharvest fungal contamination of waxberries during shipment and storage.