文献类型：期刊文献

英文题名：Comparative Transcriptome Analysis Revealed Candidate Genes Potentially Related to Desiccation Sensitivity of Recalcitrant Quercus variabilis Seeds

作者：Li, Dongxing[1] Li, Yingchao[1] Qian, Jialian[1] Liu, Xiaojuan[1] Xu, Huihui[1] Zhang, Guowei[2] Ren, Junjie[2] Wang, Libing[1] Zhang, Lu[1,3,4] Yu, Haiyan[1]

第一作者：Li, Dongxing

通信作者：Zhang, L[1];Yu, HY[1];Zhang, L[2];Zhang, L[3]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Hongya Mt State Owned Forest Farm Hebei, Yixian, Peoples R China;[3]Zhejiang Agr & Forestry Univ, Coll Landscape & Architecture, Hangzhou, Peoples R China;[4]Zhejiang Agr & Forestry Univ, Zhejiang Prov Key Lab Germplasm Innovat & Utiliza, Hangzhou, Peoples R China

年份：2021

卷号：12

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000703065100001)】；

基金：This work was supported by the National Key Research and Development Program of Ministry of Science and Technology of China (No. 2017YFD0600602), the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFYBB2018ZB001), the open funding of State Key Laboratory of Tree Genetics and Breeding (TGB2019004), the Personnel Startup Project of the Scientific Research and Development Foundation of Zhejiang A&F University (2021FR041), and the Youth Top Talent Project of the Ten Thousand Talents Program of the State.

语种：英文

外文关键词：Quercus variabilis; recalcitrant seeds; desiccation sensitivity; transcriptome; differentially expressed genes; plant hormone signaling

摘要：Chinese cork oak (Quercus variabilis) is a widely distributed and highly valuable deciduous broadleaf tree from both ecological and economic perspectives. Seeds of this species are recalcitrant, i.e., sensitive to desiccation, which affects their storage and long-term preservation of germplasm. However, little is known about the underlying molecular mechanism of desiccation sensitivity of Q. variabilis seeds. In this study, the seeds were desiccated with silica gel for certain days as different treatments from 0 (Control) to 15 days (T15) with a gradient of 1 day. According to the seed germination percentage, four key stages (Control, T2, T4, and T11) were found. Then the transcriptomic profiles of these four stages were compared. A total of 4,405, 4,441, and 5,907 differentially expressed genes (DEGs) were identified in T2 vs. Control, T4 vs. Control, and T11 vs. Control, respectively. Among them, 2,219 DEGs were overlapped in the three comparison groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that these DEGs were enriched into 124 pathways, such as "Plant hormone signal transduction" and "Glycerophospholipid metabolism". DEGs related to hormone biosynthesis and signal transduction (ZEP, YUC, PYR, ABI5, ERF1B, etc.), stress response proteins (LEA D-29, HSP70, etc.), and phospholipase D (PLD1) were detected during desiccation. These genes and their interactions may determine the desiccation sensitivity of seeds. In addition, group specific DEGs were also identified in T2 vs. Control (PP2C62, UNE12, etc.), T4 vs. Control (WRKY1-like, WAK10, etc.), and T11 vs. Control (IBH1, bZIP44, etc.), respectively. Finally, a possible work model was proposed to show the molecular regulation mechanism of desiccation sensitivity in Q. variabilis seeds. This is the first report on the molecular regulation mechanism of desiccation sensitivity of Q. variabilis seeds using RNA-Seq. The findings could make a great contribution to seed storage and long-term conservation of recalcitrant seeds in the future.