文献类型：期刊文献

中文题名：发根农杆菌诱导牡丹生根的初步研究

英文题名：Preliminary Study on Hairy Root Occurrence of Peony Induced by Agrobactrium rhizogenes

作者：杨至德[1] 王雁[2] 刘雪梅[1] 缪崑[2] 汤巧香[1]

第一作者：杨至德

机构：[1]天津城市建设学院规划与建筑系;[2]中国林业科学研究院林业研究所国家林业局林木培育重点实验室

年份：2007

卷号：20

期号：2

起止页码：292-295

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家林业局948项目(2004-4-12)的部分研究内容

语种：中文

中文关键词：发根农杆菌;牡丹;侵染;组织培养;毛状根

外文关键词：Agrobactrium rhizogenes; peony; infect; tissue culture; hairy root

分类号：S685.11

摘要：The paper explored the possibility of hairy root occurrence of peony induced by Agrobactrium rhizogenes.It showed that R1000 had the highest hairy root inducement with a hairy root rate of 35%,whereas strains A4,MSU-2 and MT232 had little difference in hairy root occurrence.Infect conditions had great influence on hairy root inducement,and the better ones are: acetosyringone concentration,30 mg·L-1;infection time,15 minutes;and culturing conditions of strains,temperature 28 ℃,28 hours shaking culture under dark environment with shaking.Pre-experiment on tissue culture system of peony was conducted,and got better medium composition as: WP+6-BA 0.5 mg·L-1+ IBA 0.1 mg·L-1+GA30.5 mg·L-1for base buds,and MS + 6-BA 2.0 mg·L-1+IBA 0.1 mg·L-1+GA3 0.5 mg·L-1for stem segments.
The paper explored the possibility of hairy root occurrence of peony induced by Agrobactrium rhizogenes. It showed that R1000 had the highest hairy root inducement with a hairy root rate of 35%, whereas strains A4, MSU-2 and MT232 had little difference in hairy root occurrence. Infect conditions had great influence on hairy root inducement, and the better ones are： acetosyringone concentration, 30 mg· L^-1; infection time, 15 minutes; and culturing conditions of strains, temperature 28 ℃, 28 hours shaking culture under dark environment with shaking. Pre-experiment on tissue culture system of peony was conducted, and got better medium composition as ： WP ＋ 6-BA 0.5 mg·L^-1＋ IBA0.1 mg·L^-1＋GA30.5 mg· L^-1 for base buds, and MS ＋ 6-BA2.0mg· L^-1＋IBA0.1 mg· L^ -1 ＋ GA3 0.5 mg · L ^-1for stem segments.