文献类型：期刊文献

英文题名：Molecular response of poplar to single and combined ozone and drought

作者：Zhang, Jin[1,2,3] Gao, Feng[1] Jia, Huixia[2] Hu, Jianjun[2] Feng, Zhaozhong[1]

第一作者：Zhang, Jin

通信作者：Feng, ZZ[1];Hu, JJ[2]

机构：[1]Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, State Key Lab Tree Genet & Breeding,Key Lab Tree, Beijing 100091, Peoples R China;[3]Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA

年份：2019

卷号：655

起止页码：1364-1375

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：;EI(收录号：20184806166825);Scopus(收录号：2-s2.0-85057327568);WOS:【SCI-EXPANDED(收录号:WOS:000455034600132)】；

基金：This study was supported by Key Research Program of Frontier Sciences, CAS (QYZDB-SSW-DQC019), and National Natural Science Foundation of China (41771034).

语种：英文

外文关键词：Climate change; Drought; Ground-level ozone; Poplar; Transcriptome; Helitrons; Protein-protein interaction (PPI) network

摘要：High concentration in ground-level ozone (O-3) and water deficit affect forest ecosystems service. Previously we found intercellular CO2 concentration and isoprene emission were affected by the combination of O-3 and drought, but the molecular mechanisms controlling these phenotypes are still open questions. In this study, we investigated the stomatal conductance (g(s)) and transcriptome changes in an O-3-sensitive hybrid poplar exposed to two O-3 levels [charcoal-filtered ambient air (CF) and non-filtered ambient air plus 40 ppb (NF40)] and two water conditions [well-watered (W) and moderate drought (D)]. NF40 reduced the gs more under D than W. We identified the differentially expressed genes (DEGs) from pairwise comparisons and found the poplar's molecular response to drought was counteracted by elevated O-3. From nine clusters obtained through K-means clustering, 12 core transcription factors were identified. DEGs involved in isoprene biosynthesis and phytohormones signal pathways indicate the molecular response and stomatal closure of poplar under O-3 and/or drought might be through MEP/DOXP and ABA-dependent pathways. In addition, 102 Helitrons capturing DEGs were involved in response to O-3 and/or drought and related with ABA-dependent pathway. This integrated analysis provides multi-dimensional insights to understand the molecular response to the combination of O-3 and drought. (C) 2018 Elsevier B.V. All rights reserved.