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我国部分兰属植物菌根真菌rDNA ITS序列分析     被引量:27

rDNA ITS Analysis of Mycorrhizal Fungi in Cymbidium Plants

文献类型:期刊文献

中文题名:我国部分兰属植物菌根真菌rDNA ITS序列分析

英文题名:rDNA ITS Analysis of Mycorrhizal Fungi in Cymbidium Plants

作者:李潞滨[1] 胡陶[1] 唐征[2] 庄彩云[1] 刘振静[1] 杨凯[2] 彭镇华[1]

第一作者:李潞滨

机构:[1]中国林业科学研究院林业研究所国家林业局林木培育重点实验室,北京100091;[2]北京农学院农业应用新技术北京市重点实验室,北京102206

年份:2008

卷号:44

期号:2

起止页码:160-164

中文期刊名:林业科学

外文期刊名:Scientia Silvae Sinicae

收录:CSTPCD;;Scopus;北大核心:【北大核心2004】;CSCD:【CSCD2011_2012】;

基金:‘948’项目(2004-4-26);‘948’项目(2005-4-37);北京市属市管高校人才强教计划资助项目

语种:中文

中文关键词:兰属植物;菌根真菌;rDNA;ITS分析;瘤菌根菌;胶膜菌属

外文关键词:Cymbidium; mycorrhizal-fungi; rDNA internal transcribed spacer analysis; Epulorhiza; Tulasnella

分类号:S718.81

摘要:rDNA internal transcribed spacer analysis(rDNA ITS)was used to study diversity of 12 representative mycorrhizal Rhizoctonia strains,which were isolated from Chinese orchids(Cymbidium)distributed in different sites and belonged to different ecologic types.The Blast results indicated that all these strains belonged to Epulorhiza or Tulasnella,which was fully consistent with identification as Epulorhiza with morphological method.Additionally,based on rDNA ITS sequence cluster analysis,dendrogram of Cymbidium mycorrhizal strains showed that the distributed environments and orchids species were two crucial factors that affected the specificity-relation between Chinese orchids(Cymbidium)symbiotic mycorrhizae and hosts.
rDNA internal transcribed spacer analysis (rDNA ITS) was used to study diversity of 12 representative mycorrhizal Rhizoctonia strains, which were isolated from Chinese orchids ( Cymbidium ) distributed in different sites and belonged to different ecologic types. The Blast results indicated that all these strains belonged to Epulorhiza or Tulasnella, which was fully consistent with identification as Epulorhiza with morphological method. Additionally, based on rDNA ITS sequence cluster analysis, dendrogram of Cymbidium mycorrhizal strains showed that the distributed environments and orchids species were two crucial factors that affected the specificity-relation between Chinese orchids (Cymbidium) symbiotic mycorrhizae and hosts.

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