文献类型：期刊文献

英文题名：Examination of the Transcriptional Response to LaMIR166a Overexpression in Larix kaempferi (Lamb.) Carr

作者：Fan, Yanru[1,2] Li, Wanfeng[1,2] Li, Zhexin[3] Dang, Shaofei[1,2] Han, Suying[4] Zhang, Lifeng[1,2] Qi, Liwang[1,2]

通信作者：Zhang, LF[1];Qi, LW[1];Zhang, LF[2];Qi, LW[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Natl Forestry & Grassland Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China;[3]Chongqing Univ Arts & Sci, Coll Landscape Architecture & Life Sci, Chongqing Key Lab Econ Plant Biotechnol, Inst Special Plants, Chongqing 402160, Peoples R China;[4]Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Beijing 100091, Peoples R China

年份：2021

卷号：10

期号：7

外文期刊名：BIOLOGY-BASEL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000686201200001)】；

基金：This research was funded by the National Natural Science Foundation of China, grant numbers 31330017, 31600544, and 31770714; the National Transgenic Major Program of China, grant number 2018ZX08020-003; and the Fundamental Research Funds for the Central Non-profit Research Institution of CAF, grant number CAFYBB2019SY011.

语种：英文

外文关键词：miR166a; HD-ZIP III; Larix kaempferi (Lamb; ) Carr; transcriptome; correlation analysis

摘要：Simple Summary The study of somatic embryogenesis can provide insights into early plant development. To elucidate the molecular mechanisms associated with miR166 in Larix kaempferi (Lamb.) Carr, the transcriptional profiles of wild-type (WT) and LaMIR166a-overexpressing embryonic cells were subjected to RNA sequencing. In total, 2467 differentially expressed genes were obtained. The cleaved degree of LaHDZ31-34 was higher in transgenic lines than in WT. The genes related to LaHDZ31-34 were screened by transcriptome analysis, and a yeast one-hybrid and dual-luciferase report assay revealed that LaHDZ31-34 could bind to the promoters of LaPAP, LaPP1, LaZFP5, and LaPHO1. This study provides insights into the regulatory mechanisms of miR166. The study of somatic embryogenesis can provide insight into early plant development. We previously obtained LaMIR166a-overexpressing embryonic cell lines of Larix kaempferi (Lamb.) Carr. To further elucidate the molecular mechanisms associated with miR166 in this species, the transcriptional profiles of wild-type (WT) and three LaMIR166a-overexpressing transgenic cell lines were subjected to RNA sequencing using the Illumina NovaSeq 6000 system. In total, 203,256 unigenes were generated using Trinity de novo assembly, and 2467 differentially expressed genes were obtained by comparing transgenic and WT lines. In addition, we analyzed the cleaved degree of LaMIR166a target genes LaHDZ31-34 in different transgenic cell lines by detecting the expression pattern of LaHdZ31-34, and their cleaved degree in transgenic cell lines was higher than that in WT. The downstream genes of LaHDZ31-34 were identified using Pearson correlation coefficients. Yeast one-hybrid and dual-luciferase report assays revealed that the transcription factors LaHDZ31-34 could bind to the promoters of LaPAP, LaPP1, LaZFP5, and LaPHO1. This is the first report of gene expression changes caused by LaMIR166a overexpression in Japanese larch. These findings lay a foundation for future studies on the regulatory mechanism of miR166.