文献类型：期刊文献

英文题名：Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches

作者：Xu, Hui[1,2] Li, Zhen[3,4] Jiang, Peng-Fei[1,2] Zhao, Li[5] Qu, Chang[1] Van de Peer, Yves[3,4,6] Liu, Yan-Jing[1] Zeng, Qing-Yin[1,2]

第一作者：Xu, Hui

通信作者：Liu, YJ[1];Zeng, QY[1];Zeng, QY[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China;[3]Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium;[4]VIB Ctr Plant Syst Biol, B-9052 Ghent, Belgium;[5]Umea Univ, Dept Ecol & Environm Sci, SE-90187 Umea, Sweden;[6]Univ Pretoria, Dept Biochem Genet & Microbiol, ZA-0028 Pretoria, South Africa

年份：2022

卷号：110

期号：1

起止页码：129-146

外文期刊名：PLANT JOURNAL

收录：;EI(收录号：20220811699993);Scopus(收录号：2-s2.0-85125078290);WOS:【SCI-EXPANDED(收录号:WOS:000759059500001)】；

基金：This study was supported by the National Science Foundation of China (31822011 and 31425006) and the Chinese Academy of Forestry (CAFYBB2018ZX001). ZL is funded by a post-doctoral fellowship from the Special Research Fund of Ghent University (BOFPDO2018001701).

语种：英文

外文关键词：active site motif; gene family; functional divergence; glutaredoxin; enzymatic specificity; Populus trichocarpa

摘要：Enzymes are essential components of all biological systems. The key characteristics of proteins functioning as enzymes are their substrate specificities and catalytic efficiencies. In plants, most genes encoding enzymes are members of large gene families. Within such families, the contributions of active site motifs to the functional divergence of duplicate genes have not been well elucidated. In this study, we identified 41 glutaredoxin (GRX) genes in the Populus trichocarpa genome. GRXs are ubiquitous enzymes in plants that play important roles in developmental and stress tolerance processes. In poplar, GRX genes were divided into four classes based on clear differences in gene structure and expression pattern, subcellular localization, enzymatic activity, and substrate specificity of the encoded proteins. Using site-directed mutagenesis, this study revealed that the divergence of the active site motif among different classes of GRX proteins resulted in substrate switches and thus provided new insights into the molecular evolution of these important plant enzymes.