文献类型：期刊文献

英文题名：The phased chromosome-scale genome of yellowhorn sheds light on the mechanism of petal color change

作者：Bi, Quanxin[1] Wang, Mengke[1] Li, Jiang[2] Lian, Jinmin[2] Niu, Yongchao[2] Cui, Yifan[1] Liu, Xiaojuan[1] Yu, Haiyan[1] Wang, Libing[1]

第一作者：毕泉鑫

通信作者：Wang, LB[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Biozeron Shenzhen Inc, Shenzhen 518000, Peoples R China

年份：2023

卷号：9

期号：6

起止页码：1193-1206

外文期刊名：HORTICULTURAL PLANT JOURNAL

收录：;Scopus(收录号：2-s2.0-85172775068);WOS:【SCI-EXPANDED(收录号:WOS:001107449300001)】；

基金：Acknowledgements This work was fi nancially supported by the Central Public-Interest Scienti fi c Institution Basal Research Fund (Grant No. CAFYBB2020QB001) and the National Natural Science Foundation of China (Grant No. 31800571) .

语种：英文

外文关键词：Xanthoceras sorbifolium var. purpurea; Phased genome; Metabolomics; Transcriptomics; Petal color

摘要：Yellowhorn (Xanthoceras sorbifolium), especially its varieties, is the red petal yellowhorn (X. sorbifolium var. purpurea), an important tree species with great ornamental value, and its flower petals change color throughout the flowering period. In this study, we mainly focused on the mechanism of the petal color change with transcriptomics and metabolomics data. A phased chromosome-scale assembly of the red petal yellowhorn genome was generated using the PacBio high-fidelity reads, Illumina short reads, and Phase genomics Proximo Hi-C data. The final de novo assembly yielded 533.67 Mb with a contig N50 of 5.42 Mb, and 27 501 protein-coding genes were predicted. Notably, an alternate haplotig assembly was also obtained. Furthermore, a variation database for the alleles within the genome was constructed. Subsequently, the expression pattern of flower pigmentation-related genes and allelic expression imbalance events were investigated. Apart from 6 genes involved in the anthocyanin biosynthesis pathway regulated by the activation of 15 MYB-bHLH-WD40s, the increased expression of senescence -related gene 1 (SRG1) and 2-oxoglutarate-dependent dioxygenase (DIOX5) might also result in decreasing content of lutein and increasing abundance of (E/Z)-phytoene, cyanidin-3-O-rutinoside, and cyanidin-3-O-sambubioside. These changes in genes and metabolites were most likely related to the petal color change in red petal yellowhorn. This phased chromosome-scale genome assembly provides more accurate genomic information for future molecular breeding and facilitates allele function studies of the red petal yellowhorn.