文献类型：期刊文献

英文题名：Trace Immunosensing of Multiple Neonicotinoid Insecticides by a Novel Broad-Specific Antibody Obtained from a Rational Screening Strategy

作者：Jiao, Shasha[1] Wang, Yan[1] Chang, Yunyun[1] Liu, Pengyan[1] Chen, Yang[1] Liu, Yihua[2] Zhu, Guonian[1] Guo, Yirong[1]

第一作者：Jiao, Shasha

通信作者：Guo, YR[1]

机构：[1]Zhejiang Univ, Key Lab Mol Biol Crop Pathogens & Insects, Minist Agr, Inst Pesticide & Environm Toxicol,Key Lab Biol Cr, Hangzhou 310058, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China

年份：2022

卷号：12

期号：9

外文期刊名：BIOSENSORS-BASEL

收录：;EI(收录号：20231213760878);Scopus(收录号：2-s2.0-85138347000);WOS:【SCI-EXPANDED(收录号:WOS:000860103400001)】；

基金：This work was supported by the National Natural Science Foundation of China (31871994) and the Key Research and Development Program in Zhejiang Province (2020C02024).

语种：英文

外文关键词：neonicotinoid pesticides; broad-specific mAb; immunochromatography; rapid detection

摘要：Residues of neonicotinoid pesticides have potential risks to food, environmental and biological safety. In this study, the hapten toward imidacloprid was adopted to gain antibodies. After molecular modeling, the electrostatic potentials of eight commonly-used neonicotinoid pesticides were individually calculated to analyze the structural similarity. Two representative compounds (imidacloprid and acetamiprid) with moderate similarity were rationally selected for hybridoma screening. Using this strategy, four clones of broad-specific monoclonal antibodies (mAbs) against multiple neonicotinoids were obtained, and the clone 6F11 exhibited the broadest spectrum to six neonicotinoid pesticides and two metabolites, with half-maximal inhibitory concentrations (IC50) ranging from 0.20 to 5.92 ng/mL. Then, the novel antibody gene was sequenced and successfully expressed in full-length IgG form using mammalian cells. Based on the sensitive recombinant antibody, a gold lateral-flow immunosensing strip assay was developed and it was qualified for rapid detection of imidacloprid, clothianidin or imidaclothiz residues in food samples.