文献类型：期刊文献

英文题名：Transcriptomic and metabolomic insights into the adaptive response of Salix viminalis to phenanthrene

作者：Li Xia[1,2] Ma Xiaodong[1] Cheng Yunhe[3] Liu Junxiang[1] Zou Junzhu[1] Zhai Feifei[4] Sun Zhenyuan[1] Han Lei[1]

第一作者：Li Xia

通信作者：Han, L[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry A, Res Inst Forestry, Beijing 100091, Peoples R China;[2]Heze Univ, Coll Agr & Bioengn, Peony Inst, Heze 274000, Shandong, Peoples R China;[3]Beijing Acad Forestry & Pomol Sci, Beijing 100093, Peoples R China;[4]Henan Polytech Univ, Sch Architectural & Artist Design, Jiaozuo 454000, Henan, Peoples R China

年份：2021

卷号：262

外文期刊名：CHEMOSPHERE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000587290300005)】；

基金：This work was jointly supported by the Special Fund for Basic Scientific Research Operating Expenses of the Central Public Welfare Research Institutes "Introduction and Application Model of Shrubs and Willows for Water Purification in Xiongan New Area" (No. CAFYBB2018ZB002) and the National Natural Science Foundation of China (No. 31700533).

语种：英文

外文关键词：Defensive mechanisms; Metabolic pathways; Phenanthrene; Salix viminalis

摘要：Polycyclic aromatic hydrocarbons (PAHs) are widespread, persistent environmental pollutants. They exert toxic effects at different developmental stages of plants. Plant defense mechanisms against PAHs are poorly understood. To this end, transcriptomics and widely targeted metabolomic sequencing were used to study the changes in gene expression and metabolites that occur in the roots of Salix viminalis subjected to phenanthrene stress. Significant variations in genes and metabolites were observed between treatment groups and the control group. Thirteen amino acids and key genes involved in their biosynthesis were upregulated exposed to phenanthrene. Cysteine biosynthesis was upregulated. Sucrose, inositol galactoside, and mellidiose were the main carbohydrates that were largely accumulated. Glutathione biosynthesis was enhanced in order to scavenge reactive oxygen species and detoxify the phenanthrene. Glucosinolate and flavonoid biosynthesis were upregulated. The production of pinocembrin, apigenin, and epigallocatechin increased, which may play a role in antioxidation to resist phenanthrene stress. In addition, levels of six amino acids and N,N'-(p-coumaroyl)-cinnamoyl-caffeoyl-spermidine were significantly increased, which may have helped protect the plant against phenanthrene stress. These results demonstrated that S. viminalis had a positive defense strategy in response to phenanthrene challenge. Subsequent defense-related reactions may have also occurred within 24 h of phenanthrene exposure. The findings of the present study would be useful in elucidating the molecular mechanisms regulating plant responses to PAH challenges and would help guide crop and plant breeders in enhancing PAH resistance. (C) 2020 Published by Elsevier Ltd.