文献类型：期刊文献

英文题名：Metabolomics and Transcriptomics Analyses Reveal Regulatory Networks Associated with Fatty Acid Accumulation in Pecan Kernels

作者：Zhang, Chengcai[1] Ren, Huadong[1] Yao, Xiaohua[1] Wang, Kailiang[1] Chang, Jun[1] Shao, Weizhong[2]

第一作者：张成才

通信作者：Ren, HD[1];Yao, XH[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China;[2]Forestry Bur Jiande, Jiande 311600, Zhejiang, Peoples R China

年份：2022

卷号：70

期号：50

起止页码：16010-16020

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20225013241174);Scopus(收录号：2-s2.0-85143667201);WOS:【SCI-EXPANDED(收录号:WOS:000903336300001)】；

基金：? ACKNOWLEDGMENTS This research was supported by the Fundamental Research Funds of CAF No. CAFYBB2018SY013 and the Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural Varieties (2021C02066-12) .

语种：英文

外文关键词：nut; nutrient; metabolite; gene expression; transcription factor; coexpression analysis

摘要：Pecans are a globally important tree nut crop. Pecan nuts are rich in fatty acids (FAs), proteins, and flavonoids in addition to thiamine and numerous micronutrients. Although several of these nutriments have been studied in this plant, the comprehensive metabolite variations and molecular mechanisms associated with them have not been fully elucidated. In this study, untargeted metabolomics and transcriptomics were integrated to reveal the metabolite accumulation patterns and their associated molecular mechanisms during pecan kernel development. In total, 4260 (under positive mode) and 2726 (under negative mode) high quality features were retained. Overall, 163 differentially accumulated metabolites were identified. Most components were classified into the categories "organic acids and derivatives " and "lipids and lipid-like molecules. " The accumulation patterns of amino acids, FAs, carbohydrates, organic acids, vitamins, flavonoids, and phenylpropanoids alongside embryo development were determined. Furthermore, transcriptomes from four pecan kernel developmental stages were used to assess transcript expression levels. Coexpression analyses were performed between FAs and their related genes. This study provides a comprehensive overview of the metabolic changes and regulations during pecan kernel development. We believe that the identification of nutriment accumulation trends and hub genes associated with the biosynthesis of the components will be valuable for genetically improving this plant.