文献类型：期刊文献

英文题名：AtBET5 is essential for exine pattern formation and apical meristem organization in Arabidopsis

作者：Zhang, Jin[1,3] Chen, Jun[1,2] Wang, Lijuan[1] Zhao, Shutang[1] Li, Jianbo[1] Liu, Bobin[1] Li, Hongying[1] Qi, Xingyun[2] Zheng, Huanquan[2] Lu, Mengzhu[1]

第一作者：Zhang, Jin

通信作者：Lu, MZ[1];Zheng, HQ[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,State Forestry A, Beijing 100091, Peoples R China;[2]McGill Univ, Dept Biol, 1205 Dr Penfield Ave, Montreal, PQ H3A 1B1, Canada;[3]Oak Ridge Natl Lab, Biosci Div, POB 2009, Oak Ridge, TN 37831 USA

年份：2018

卷号：274

起止页码：231-241

外文期刊名：PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000442068200026)】；

基金：We thank Dr. Jian Xu (National University of Singapore) for providing pWOX5::GFP, and Dr. Xiaojiao Han (State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry) for help in sectioning. This work was supported by the Basic Research Fund of RIF (RIF2012-02) and the National Natural Science Foundation of China (31270699) to J.C. and a grant from The National Science and Engineering Research Council of Canada and a startup grant from McGill University (Montreal, Canada) to H.Z.

语种：英文

外文关键词：Arabidopsis thaliana; BETS; TRAPP; Apical meristem; Auxin; PIN1/2

摘要：BETS is a component of trafficking protein particle (TRAPP) which has been studied extensively in non-plant organisms where they are involved in membrane trafficking within Golgi and between Golgi and early endosomes. Recent analysis of TRAPP in different classes of organisms indicates that TRAPP function might exhibit differences among organisms. A single copy of the BETS gene named AtBET5 was found in the Arabidopsis genome based on sequence similarity. Developmental phenotype and the underlying mechanisms have been characterized upon transcriptional knock-down lines generated by both T-DNA insertion and RNAi. Pollen grains of the T-DNA insertional line present reduced fertility and pilate exine instead of tectate exine. Perturbation of the AtBET5 expression by RNAi leads to apical meristematic organization defects and reduced fertility as well. The reduced fertility was due to the pollination barrier caused by an altered composition and structure of pollen walls. Auxin response in root tip cells is altered and there is a severe disruption in polar localization of PIN1-GFP, but to a less extent of PIN2-GFP in the root tips, which causes the apical meristematic organization defects and might also be responsible for the secretion of sporopollenin precursor or polar targeting of sporopollenin precursor transporters.