文献类型：期刊文献

英文题名：Improving Sedum plumbizincicola genetic transformation with the SpGRF4-SpGIF1 gene and the self-excision CRE/LoxP system

作者：Zhang, Yixin[1,2,3] Mo, Yanlan[2] Ren, Hongxu[1,5,6] Wu, Xiaotong[4] Han, Liyuan[3] Sun, Zhenyuan[3] Xu, Wenzhong[1,5,6]

第一作者：Zhang, Yixin

通信作者：Xu, WZ[1];Xu, WZ[2];Xu, WZ[3]

机构：[1]Chinese Acad Sci, Inst Bot, State Key Lab Plant Divers & Specialty Crops, Beijing 100093, Peoples R China;[2]Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Peoples R China;[3]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, State Forestry Adm,Res Inst Forestry, Beijing 100091, Peoples R China;[4]Southeast Univ, Sch Energy & Environm, Nanjing 210096, Peoples R China;[5]China Natl Bot Garden, Beijing 100093, Peoples R China;[6]Univ Chinese Acad Sci, Beijing 100049, Peoples R China

年份：2024

卷号：259

期号：5

外文期刊名：PLANTA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001199511300003)】；

基金：No Statement Available

语种：英文

外文关键词：Estradiol-inducible; Molecular-assisted transformation system; Regeneration; XVE system

摘要：Sedum plumbizincicola, despite being an excellent hyperaccumulator of cadmium and zinc with significant potential for soil pollution phytoremediation on farmland, has nonetheless trailed behind other major model plants in genetic transformation technology. In this study, different explants and SpGRF4-SpGIF1 genes were used to optimize the genetic transformation of S. plumbizincicola. We found that petiole and stem segments had higher genetic transformation efficiency than cluster buds. Overexpression of SpGRF4-SpGIF1 could significantly improve the genetic transformation efficiency and shorten the period of obtaining regenerated buds. However, molecular assistance with overexpression of SpGRF4-SpGIF1 leads to abnormal morphology, resulting in plant tissue enlargement and abnormal growth. Therefore, we combined SpGRF4-SpGIF1 with XVE and Cre/loxP to obtain DNA autocleavage transgenic plants induced by estradiol, thereby ensuring normal growth in transgenic plants. This study optimized the S. plumbizincicola genetic transformation system, improved the efficiency of genetic transformation, and established a self-excision molecular-assisted transformation system. This work also established the basis for studying S. plumbizincicola gene function, and for S. plumbizincicola breeding and germplasm innovation.