文献类型：期刊文献

英文题名：Waterlogging tolerance and wood properties of transgenicPopulus alba x glandulosaexpressingVitreoscillahemoglobin gene (Vgb)

作者：Li, Yiliang[1,2,3,4] Zhang, Weixi[1,2] Zhu, Wenxu[5] Zhang, Bingyu[1,2] Huang, Qinjun[1,2] Su, Xiaohua[1,2]

第一作者：Li, Yiliang

通信作者：Su, XH[1];Su, XH[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Natl Forestry & Grassland Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China;[3]Guangdong Prov Key Lab Silviculture Protect & Uti, Guangzhou 510520, Peoples R China;[4]Guangdong Acad Forestry, Guangzhou 510520, Peoples R China;[5]Shenyang Agr Univ, Coll Forestry, 120 Dongling St, Shenyang 110866, Peoples R China

年份：2021

卷号：32

期号：2

起止页码：831-839

外文期刊名：JOURNAL OF FORESTRY RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000556977800001)】；

基金：The work was supported by the Basic Research Fund of RIF (Grant No. CAFYBB2017ZA001-3) the National Natural Science Foundation of China (Grant No. 31700589) and the Forestry Genetic Breeding National Key Laboratory (Chinese Academy of Forestry Sciences) Open Project (Grant No. TGB 2013005).

语种：英文

外文关键词：Populus alba x glandulosa; vgbgene; Waterlogging stress; Growth; Microscopy

摘要：Because overexpression ofVitreoscillahemoglobin gene (Vgb) gene in plants can enhance tolerance to waterlogging, hereVgbwas inserted intoPopulus alba x glandulosato investigate its expression and effects on growth and physiological responses to waterlogging stress in the transgenic poplars. Southern blotting and RT-PCR analysis ofVgb-transgenicP. alba x glandulosashowed that theVgbgene was integrated into the genome of the V13-81 and V13-85 transgenic lines and expressed. In greenhouse waterlogging stress tests, mortality of the transgenic poplar was significant lower than that of nontransgenic plants with increasing treatment time from 2 to 22 days. The transgenic plants had higher chlorophyll content and less chloroplast damage than in the control plants. Additionally, starch accumulation increased, and growth was enhanced in the transgenic plants, suggesting that theVgb-expressing lines had improved energy reserves. Field trials of the transgenic poplar suggested thatVgbexpression promotes growth and influences wood quality. Taken together, our results suggest that the expression ofVgbcan increase the accumulation of chlorophyll and starch in the transgenic poplar, improve its ability to endure flooding, and improve growth and wood quality of the transgenic plants.