文献类型：期刊文献

中文题名：利用SRAP标记鉴别美洲黑杨及指纹图谱构建

英文题名：Identification and Fingerprinting of Populus deltoides Using SRAP Markers

作者：郭娟[1] 樊军锋[1] 梁军[2] 李薇[1]

第一作者：郭娟

机构：[1]西北农林科技大学林学院;[2]中国林业科学研究院森林生态环境与保护研究所

年份：2014

卷号：29

期号：2

起止页码：98-102

中文期刊名：西北林学院学报

外文期刊名：Journal of Northwest Forestry University

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD_E2013_2014】；

基金：国家"十二五"科技支撑专题(2012BAD19B0801)

语种：中文

中文关键词：美洲黑杨;SRAP;品种鉴别;指纹图谱

外文关键词：Populus deltoides; SRAP; variety identification; fingerprinting analysis

分类号：S792.110.4

摘要：利用SRAP分子标记对美洲黑杨1-69及与其有亲缘关系的4个美洲黑杨品种进行了分子鉴别与指纹图谱分析。结果表明：从72对SRAP引物组合中筛选出21对引物组合可以将5个品种完全区分开，21对引物共扩增出287条带，条带清晰，平均每对引物扩增条带为13．7条，多态性条带209条，多态性比率为72．89／6，多态性高；各杨树品种之间的遗传相似系数在0．5481～0．7692之间，平均相似系数为0．6587。并选用扩增出的61条特异性条带构建5个杨树品种的分子指纹图谱。聚类分析结果表明，5个美洲黑杨品种间遗传差异较小，其中陕林3号与北抗1号的遗传差异最大。
Identification and molecular fingerprinting analysis of Populus deltoides including P. deltoides. cv. ＇Lux＇（I-69/55） and its 4 affinity varieties were conducted using SRAP makers. Twenty one pairs of primers were screened from 72 primer combinations that could produce polymorphic and high quality bands, and identify 5 varieties completely. Two hundreds and eighty seven bands were amplified and 209 bands were polymorphic from these 21 pairs of primers. Each pair of primers amplified 13.7 bands on aver- age, and the percentage of polymorphic bands was 72.8%. 61 specific bands amplified were used for analy- sis of fingerprinting in the 5 varieties. The similarity coefficients of the five Populus varieties ranged from 0. 548 1 to 0. 769 2 with an average of 0. 658 7. Cluster analysis showed that the genetic differentiation a- mong these 5 P. deltoides varieties was low and P. deltoides, cv. ＂SL-3＂ and P. deltoides, cv. ＂Beikang-1（16- 27/92）＂ showed the highest genetic differentiation among five varieties.